Expression of ds^EP-822 under the control of Scer\GAL4^Dll-em212 along the distal wing margin results in wing hairs showing reverse polarity or swirl patterns around the L3-3 location (position of the most distal dorsal campaniform sensillum on vein L3). These animals show defects in the directional preferences of growing microtubules in wing cells at the L3-3 location 24 hours after puparium formation (APF); in contrast to the symmetrical microtubule polarity seen in wild-type cells, plus end-proximal microtubules become more abundant (with no variation of anterior and posterior components) in the mutant cells.

At 24 hours APF, the majority of microtubules in wing cells within the expression domain of animals co-expressing ds^Scer\UAS.cTa and ds^EP-822 under the control of Scer\GAL4^en-e16E in the posterior wing compartment align themselves along the proximodistal axis, as occurs in wild-type wing cells. Wing hair polarity in the posterior compartment is not disrupted in the mutant adults, but the adjacent anterior cells (up to 5-6 cells) misorient their hairs away from the anterior/posterior compartment boundary.

Expression of ds^EP-822 under the control of Scer\GAL4^dpp.3KK results in wing vein thickening.

Expression of ds^EP-822 under the control of Scer\GAL4^MD-638 results in reduced wing size.

Expression of ds^EP-822 under the control of Scer\GAL4^sca-C253 results in extra bristles.