FlyBase Allele Report: Dmel\Myo31DF[K2]

General Information

Symbol

Dmel\Myo31DF^K2

Species

D. melanogaster

Name

FlyBase ID

FBal0194493

Feature type

allele

Associated gene

Dmel\Myo31DF

Associated Insertion(s)

Carried in Construct

Also Known As

Myo1D^K2, myoID^K2

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Speder et al., 2006)

Progenitor genotype

Myo31DF^KG02246

(Speder et al., 2006)

Cytology

Description

Imprecise excision of the insertion in Myo31DF^KG02246, resulting in a deletion of genomic DNA which includes Myo31DF sequences.

(Speder et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

male genitalia

(Okumura et al., 2015, Petzoldt et al., 2012, Speder et al., 2006)

Detailed Description

Statement

Reference

The dextral (right-handed) 360% rotation that takes place in the male genitalia of wild type flies during pupal development is reversed (sinistral) in homozygous Myo31DF^K2 mutants. Heterozygotes are similar to wild type.

(Okumura et al., 2015)

Mutant males show sinistral male genitalia rotation (instead of the wild-type dextral rotation).

(Petzoldt et al., 2012)

Mutant males show inverted genitalia rotation compared to wild-type, showing sinistral rotation instead of dextral rotation.

(Speder et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Myo31DF^K2 has male genitalia phenotype, enhanceable by Myo61F¹

(Okumura et al., 2015)

Myo31DF^K2 has male genitalia phenotype, enhanceable by Myo61F¹/Myo95E¹

(Okumura et al., 2015)

NOT Enhanced by

Statement

Reference

Myo31DF^K2 has male genitalia phenotype, non-enhanceable by Myo95E¹

(Okumura et al., 2015)

NOT suppressed by

Statement

Reference

Myo31DF^K2 has male genitalia phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Myo95E^UAS.B

(Okumura et al., 2015)

Myo31DF^K2 has male genitalia phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Myo61F^UAS.cHa

(Okumura et al., 2015)

Other

Statement

Reference

Myo31DF^K2, Myo61F^UAS.cHa, Scer\GAL4^ptc-559.1 has male genitalia phenotype

(Okumura et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous Myo61F¹ enhances the change in direction of male genitalia rotation seen in Myo31DF^K2 mutant pupae. As in Myo31DF^K2 mutants alone, the double mutants undergo a left-handed (sinistral) rotation, rather than the 360% dextral (right-handed) rotation seen in wild type flies, but a greater proportion undergo a full 360[o] sinistral rotation in the presence of Myo61F¹.

Homozygous Myo95E¹ does not enhance the change in direction of male genitalia rotation seen in Myo31DF^K2 mutant pupae. As in Myo31DF^K2 mutants alone, the double mutants undergo a left-handed (sinistral) rotation, rather than the 360[o] dextral (right-handed) rotation seen in wild type flies.

Myo61F¹/Df(3L)BSC250 enhances the change in direction of male genitalia rotation seen in Myo31DF^K2 mutant pupae. As in Myo31DF^K2 mutants alone, the double mutants undergo a left-handed (sinistral) rotation, rather than the 360[o] dextral (right-handed) rotation seen in wild type flies, but a greater proportion undergo a full 360[o] sinistral rotation in the presence of Myo61F¹/Df(3L)BSC250.

The presence of homozygous Myo61F¹ and Myo95E¹ mutations enhances the change in direction of male genitalia rotation seen in Myo31DF^K2 mutant pupae. As in Myo31DF^K2 mutants alone, the triple mutants undergo a left-handed (sinistral) rotation, rather than the 360[o] dextral (right-handed) rotation seen in wild type flies, but a greater proportion undergo a full 360[o] sinistral rotation in the presence of Myo61F¹ and Myo95E¹ mutations.

Expression of Myo95E^Scer\UAS.B under the control of Scer\GAL4^ptc-559.1 does not suppress the change in direction of male genitalia rotation seen in Myo31DF^K2/Myo31DF^L152 mutant pupae. Mutants expressing Myo95E^Scer\UAS.B continue to exhibit the left-handed (sinistral) rotation seen in Myo31DF^K2/Myo31DF^L152 mutations, as opposed to the dextral (right-handed) rotation seen in wild type.

Expression of Myo61F^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 does not suppress the change in direction of male genitalia rotation seen in Myo31DF^K2/Myo31DF^L152 mutant pupae. Mutants expressing Myo61F^Scer\UAS.cHa continue to exhibit the left-handed (sinistral) rotation seen in Myo31DF^K2/Myo31DF^L152 mutations, as opposed to the dextral (right-handed) rotation seen in wild type. Expressing Myo61F^Scer\UAS.cHa in Myo31DF^K2 heterozygotes produces partial sinistral phenotypes.

(Okumura et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Myo31DF^L152/Myo31DF^K2 is rescued by Scer\GAL4^ptc-559.1/Myo31DF^UAS.GFP

(Okumura et al., 2015)

Comments

Expression of Myo31DF^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ptc-559.1 completely rescues the change in direction of male genitalia rotation seen in Myo31DF^K2/Myo31DF^L152 mutant pupae. Mutants expressing Myo31DF^{Scer\UAS.T:Avic\GFP} undergo the 360[o] dextral (right-handed) rotation rather than the left-handed (sinistral) rotation seen in Myo31DF^K2/Myo31DF^L152 mutations.

(Okumura et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

KG02246²

(Speder et al., 2006)

Myo1D^K2

(Lapraz et al., 2023, Amcheslavsky et al., 2018)

Myo1D^k2

(Lebreton et al., 2018)

Myo31DF^K2

(Speder et al., 2006)

MyoID^K2

(Ishibashi et al., 2019)

myo1D^K2

(Chougule et al., 2020)

myoID^K2

(Petzoldt et al., 2012)

myoID^k2

(González-Morales et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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