FlyBase Allele Report: Dmel\Myo95E[1]

General Information

Symbol

Dmel\Myo95E¹

Species

D. melanogaster

Name

FlyBase ID

FBal0316574

Feature type

allele

Associated gene

Dmel\Myo95E

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Okumura et al., 2015)

Mutagen

P-element activity

(Okumura et al., 2015)

Progenitor genotype

P{EPgy2}Myo95E^EY22671

(Okumura et al., 2015)

Cytology

Description

Imprecise excision of the P{EPgy2} element in P{EPgy2}Myo95E^EY22671 has resulted in a 2893bp deletion, removing the putative initiation codon for all six Myo95E isoforms.

(Okumura et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:24,158,588..24,161,480 [-]

Comment:

A 2893 bp deletion resulting from the imprecise excision of P{EPgy2}Myo95E^EY22671. The genome coordinates of the deletion reported by author.

(Okumura et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Okumura et al., 2015)

viable

(Okumura et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

The hatching rates and survival rates from first instar larvae to adult of homozygous Myo95E¹ mutants (derived from homozygous Myo95E¹ mutant females) are similar to those of wild type. No abnormalities are observed in the embryonic and adult organs of these flies.

The hatching rates and survival rates from first instar larvae to adult of Myo95E¹/Df(3R)Exel6198 transheterozygotes (derived from homozygous Myo95E¹ mutant females) are similar to those of wild type.

Homozygous Myo95E¹ mutants show normal left/right (l/r) asymmetric development in all sections of the embryonic gut.

As in wild type, the 360[o] rotation that takes place in the male genitalia of homozygous Myo95E¹ mutant flies during pupal development occurs in the dextral (right-handed) direction.

(Okumura et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Myo95E¹ is a non-enhancer of abnormal axis specification phenotype of Myo31DF^L152

(Okumura et al., 2015)

Myo61F¹/Myo95E¹ is a non-enhancer of abnormal axis specification phenotype of Myo31DF^L152

(Okumura et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Myo61F¹/Myo95E¹ is an enhancer of male genitalia phenotype of Myo31DF^K2

(Okumura et al., 2015)

NOT Enhancer of

Statement

Reference

Myo95E¹ is a non-enhancer of embryonic/larval hindgut phenotype of Myo31DF^L152

(Okumura et al., 2015)

Myo95E¹ is a non-enhancer of embryonic/larval posterior midgut phenotype of Myo31DF^L152

(Okumura et al., 2015)

Myo61F¹/Myo95E¹ is a non-enhancer of embryonic/larval hindgut phenotype of Myo31DF^L152

(Okumura et al., 2015)

Myo61F¹/Myo95E¹ is a non-enhancer of embryonic/larval posterior midgut phenotype of Myo31DF^L152

(Okumura et al., 2015)

Myo95E¹ is a non-enhancer of male genitalia phenotype of Myo31DF^K2

(Okumura et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Mutants that are doubly homozygous for Myo61F¹ and Myo95E¹ show normal left/right (l/r) asymmetric development in all sections of the embryonic gut.

Neither homozygous Myo95E¹ nor homozygous Myo61F¹ enhances the frequency of left/right (l/r) inversion in the posterior midgut and the hindgut seen in homozygous Myo31DF^L152 mutant embryos. As in homozygous Myo31DF^L152 mutants alone, ~80% of the double mutants exhibit the l/r reversed phenotype. No further enhancement is seen in the homozygous Myo95E¹, Myo61F¹ and Myo31DF^L152 triple mutants.

As in wild type, the 360[o] rotation that takes place in the male genitalia of double homozygotic Myo95E¹ Myo61F¹ mutant flies during pupal development occurs in the dextral (right-handed) direction.

Homozygous Myo95E¹ does not enhance the change in direction of male genitalia rotation seen in Myo31DF^K2 mutant pupae. As in Myo31DF^K2 mutants alone, the double mutants undergo a left-handed (sinistral) rotation, rather than the 360[o] dextral (right-handed) rotation seen in wild type flies.

The presence of homozygous Myo61F¹ and Myo95E¹ mutations enhances the change in direction of male genitalia rotation seen in Myo31DF^K2 mutant pupae. As in Myo31DF^K2 mutants alone, the triple mutants undergo a left-handed (sinistral) rotation, rather than the 360[o] dextral (right-handed) rotation seen in wild type flies, but a greater proportion undergo a full 360[o] sinistral rotation in the presence of Myo61F¹ and Myo95E¹ mutations.

(Okumura et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Myo95E¹

(Okumura et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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