FlyBase Allele Report: Dmel\5-HT1B[RNAi.UAS]

General Information

Symbol

Dmel\5-HT1B^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0194589

Feature type

allele

Associated gene

Dmel\5-HT1B

Associated Insertion(s)

Carried in Construct

P{UAS-5-HT1B.RNAi}

Also Known As

UAS-1BRNAi

Key Links

Transgenic product class

RNAi_reagent

(Yuan et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Yuan et al., 2005)

Mutagen

in vitro construct

(Yuan et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-5-HT1B.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a 585bp cDNA fragment (nucleotides 571-1156) cloned in one orientation and then in the reverse orientation to generate a hairpin with a small intronic sequence in between.

(Yuan et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

5-HT1B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^5-HT1B.PY

(Yuan et al., 2005)

abnormal circadian rhythm, with Scer\GAL4^elav-C155

(Yuan et al., 2005)

abnormal circadian rhythm, with Scer\GAL4^tim.PE

(Yuan et al., 2005, Yuan et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of 5-HT1B^IR.Scer\UAS under the control of Scer\GAL4^tim.PE does not affect either the total amount of sleep or sleep consolidation, measured as the average length of sleep bouts.

(Yuan et al., 2006)

Flies in which 5-HT1B^IR.Scer\UAS is expressed under the control of Scer\GAL4^tim.PE exhibit significantly increased light-induced phase shifts at low light intensity (250 lux) as compared to wild-type. In addition, 5-HTP treatment does not inhibit a strong light response in these flies.

(Yuan et al., 2005)

Expression of 5-HT1B^IR.Scer\UAS, under the control of Scer\GAL4^5-HT1B in flies that have been subjected to light pulses of different intensities results in significantly increased phase shifts as compared to wild-type flies. These flies are also immune to 5-HTP treatment.

Flies in which 5-HT1B^IR.Scer\UAS is expressed under the control of Scer\GAL4^tim.PE show significantly increased light-induced phase shifts at low light intensity as compared to control flies. In addition, 5-HTP treatment does not inhibit the light response of these flies.

Expression of 5-HT1B^IR.Scer\UAS specifically in cells that normally express 5-HT1B (i.e. under the control of Scer\GAL4^5-HT1B) results in circadian periods significantly longer than those of controls. The same effect is observed when 5-HT1B^IR.Scer\UAS is expressed under the control of Scer\GAL4^elav-C155.

(Yuan et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

5-HT1B^RNAi.UAS, 5-HT1B^UAS.cYa, Scer\GAL4^tim.PE has abnormal circadian rhythm phenotype, suppressible by cry^b, Scer\GAL4^tim.PE

(Yuan et al., 2005)

Suppressor of

Statement

Reference

5-HT1B^RNAi.UAS, Scer\GAL4^tim.PE is a suppressor of abnormal circadian rhythm phenotype of Scer\GAL4^tim.PE, cry^UAS.cDa, cry^b

(Yuan et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of 5-HT1B^IR.Scer\UAS, under the control of RU486-inducible Scer\GAL4^{Mef2.247.Switch} in a 5-HT1A^Δ5kb heterozygous background does not produce any baseline sleep phenotype.

(Yuan et al., 2006)

Reducing 5-HT1B expression, through induction of 5-HT1B^IR.Scer\UAS, under the control of Scer\GAL4^tim.PE, eliminates the strong behavioural rhythms in constant bright light observed in cry^b heterozygous flies overexpressing 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE.

(Yuan et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

27634

y¹ w^*; P{UAS-5-HT1B.RNAi}2

27635

y¹ w^*; P{UAS-5-HT1B.RNAi}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

5-HT1B^IR.Scer\UAS

5-HT1B^RNAi.UAS

5-HT1B^{dsRNA.Scer\UAS}

5-HT1B^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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