The Tag:TAP-FLAG coding region (Spe I fragment) is inserted just prior to the stck stop codon within a genomic construct encompassing both the endogenous stck upstream regulatory sequence and the coding region.
stckTag:TAP-FLAG rescues stck18/Df(3R)097
Carried in plasmid "pCaspin-TAP" and used to purify soluble, cytoplasmic stckT:Zzzz\TAP-FLAG protein complexes from Drosophila embryos.
Carried in plasmid "pCaspin-TAP" and used to purify soluble, cytoplasmic stckT:Zzzz\TAP-FLAG protein complexes from Drosophila embryos.