FlyBase Allele Report: Dmel\stck[18]

General Information

Symbol

Dmel\stck¹⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0150234

Feature type

allele

Associated gene

Dmel\stck

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Clark et al., 2003)

Mutagen

Progenitor genotype

Cytology

Description

2bp deletion in stck removing nucleotides 2309-2310.

(Clark et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | embryonic stage (with stck^3R-17)

(Elias et al., 2012)

lethal | larval stage (with stck^3R-17)

(Elias et al., 2012)

viable (with stck^3R-17), with stck^{D303V.Tag:FLAG}

(Pronovost et al., 2013)

visible (with stck^3R-17), with stck^{D303V.Tag:FLAG}

(Pronovost et al., 2013)

Phenotype Manifest In

embryonic/first instar larval cuticle | dorsal | germline clone

(Kadrmas et al., 2004)

embryonic somatic muscle cell (with Df(3R)097)

(Clark et al., 2003)

myofibril & actin filament (with Df(3R)097)

(Clark et al., 2003)

wing (with stck^3R-17), with stck^{D303V.Tag:FLAG}

(Pronovost et al., 2013)

Detailed Description

Statement

Reference

stck^3R-17/stck¹⁸ adults rescued by stck^{D303V.T:Zzzz\FLAG} are recovered at 90% of the expected frequency. The rescued adults frequently have wing blisters and wings are often seen in a "held up" position.

(Pronovost et al., 2013)

Embryos expressing stck^{wt.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background do not display any muscle structure or actin cytoskeleton defects.

Embryos expressing stck^{Q38A.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background do not display any muscle structure or actin cytoskeleton defects.

(Elias et al., 2012)

stck¹⁸/Df(3R)097 embryos show a disruption in muscle fibre morphology; the muscles are less organised than normal and gaps are occasionally seen between adjacent muscle cells, indicating a failure of some muscle attachment sites. Early stage 17 mutant muscles have defects in actin filament organisation; the actin filament bundles of the myofibrils are buckled in appearance and often do not extend to the segment boundaries. In addition, many of the muscle attachments lack the enrichment of filamentous actin seen in wild-type animals.

(Clark et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

stck[+]/stck¹⁸ is a suppressor of partially lethal - majority die | recessive phenotype of msn⁰³³⁴⁹

(Kadrmas et al., 2004)

Other

Statement

Reference

ics^BG02577, stck¹⁸/stck^3R-17, stck^{Q38A.Tag:FLAG} has partially lethal - majority die phenotype

(Elias et al., 2012)

ics^BG02577, stck¹⁸/stck^3R-17, stck^{Q38A.Tag:FLAG} has abnormal developmental rate | first instar larval stage phenotype

(Elias et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

stck[+]/stck¹⁸ is a suppressor of adult thorax phenotype of msn⁰³³⁴⁹

(Kadrmas et al., 2004)

stck[+]/stck¹⁸ is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of msn¹⁰²

(Kadrmas et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

ics^BG02577 has no effect on the viability of flies expressing stck^{wt.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background.

The addition of ics^BG02577 reduces the viability of flies expressing stck^{Q38A.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background. Between 6 and 34% of the expected number of progeny are seen, with the majority of the lethality taking place during the larval and pharate stages. The rescued larvae display a range of sizes, including presumptive first instar larvae that appear stumpy, exhibit sluggish movement and do not progress in development.

(Elias et al., 2012)

Introduction of a single copy of stck¹⁸ into the homozygous msn¹⁰² background reduces the incidence of a 'dorsal open' phenotype from approximately 30% to approximately 20%.

Introduction of a single copy of stck¹⁸ into the homozygous msn⁰³³⁴⁹ homozygous background reduces the incidence of eclosion from approximately 0% to approximately 15% and also suppresses the msn⁰³³⁴⁹ thorax closure defect.

(Kadrmas et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

stck¹⁸/stck^3R-17 is rescued by stck^Tag:FLAG

(Yoshigi et al., 2013)

stck¹⁸/stck^3R-17 is rescued by stck^wt.Tag:FLAG

(Elias et al., 2012)

stck¹⁸/stck^3R-17 is rescued by stck^{Q38A.Tag:FLAG}

(Elias et al., 2012)

stck¹⁸/Df(3R)097 is rescued by stck^EGFP

(Kadrmas et al., 2004)

stck¹⁸/Df(3R)097 is rescued by stck^Tag:TAP-FLAG

(Kadrmas et al., 2004)

Partially rescued by

stck¹⁸/stck^3R-17 is partially rescued by stck^{D303V.Tag:FLAG}

(Pronovost et al., 2013)

Comments

Expression of stck^{wt.T:Zzzz\FLAG} rescues the embryonic and larval lethality seen in stck^3R-17/stck¹⁸ mutants. The rescued flies do not display any overt phenotypes.

Expression of stck^{Q38A.T:Zzzz\FLAG} rescues the embryonic and larval lethality seen in stck^3R-17/stck¹⁸ mutants. The rescued flies are fertile and do not display either wing blisters or any other overt phenotypes.

(Elias et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66570

y¹ w¹; P{neoFRT}82B stck¹⁸ P{white-un1}90E/TM3, P{ActGFP}JMR2, Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

stck¹⁸

(Pronovost et al., 2013, Yoshigi et al., 2013, Elias et al., 2012, Kadrmas et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (5)

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