FlyBase Allele Report: Dmel\stck[wt.Tag:FLAG]

General Information

Symbol

Dmel\stck^wt.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0278273

Feature type

allele

Associated gene

Dmel\stck

Associated Insertion(s)

Carried in Construct

P{stck-FLAG.wt}

Key Links

Transgenic product class

genomic_DNA

(Elias et al., 2012)

wild_type

(Elias et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Elias et al., 2012)

wild_type

(Elias et al., 2012)

Mutagen

in vitro construct

(Elias et al., 2012)

Progenitor genotype

P{PINCH-EGFP}

(Elias et al., 2012)

Carried in construct

P{stck-FLAG.wt}

Cytology

Description

The endogenous stck promoter drives expression of Tag:FLAG-tagged stck protein.

(Elias et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

stck

Encoded product / tool

stck

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of stck^{wt.T:Zzzz\FLAG} in an otherwise wild-type background has no effect on larval size.

(Pronovost et al., 2013)

No obvious phenotypes are seen when stck^{wt.T:Zzzz\FLAG} is expressed in wild type flies.

Embryos expressing stck^{wt.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background do not display any muscle structure or actin cytoskeleton defects.

(Elias et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

stck^wt.Tag:FLAG is a suppressor of abnormal locomotor behavior | dominant | adult stage phenotype of Mhc^S1

(Pronovost et al., 2013)

stck^wt.Tag:FLAG is a suppressor of lethal phenotype of flw⁶

(Pronovost et al., 2013)

stck^wt.Tag:FLAG is a suppressor of lethal phenotype of flw^G0172

(Pronovost et al., 2013)

stck^wt.Tag:FLAG is a suppressor of majority die during larval stage phenotype of flw^G0172

(Pronovost et al., 2013)

NOT Suppressor of

Statement

Reference

stck^wt.Tag:FLAG is a non-suppressor of some die during pupal stage phenotype of flw^G0172

(Pronovost et al., 2013)

Other

Statement

Reference

flw^G0172, stck^wt.Tag:FLAG has increased body size phenotype

(Pronovost et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

stck^wt.Tag:FLAG is a suppressor of adult thorax | dorsal phenotype of Mhc^S1

(Pronovost et al., 2013)

stck^wt.Tag:FLAG is a suppressor of ventral intersegmental muscle cell | larval stage phenotype of flw^G0172

(Pronovost et al., 2013)

NOT Suppressor of

Statement

Reference

stck^wt.Tag:FLAG is a non-suppressor of indirect flight muscle cell phenotype of Mhc^S1

(Pronovost et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

stck^{wt.T:Zzzz\FLAG} increases the frequency of male flw⁶ escapers from 2.4% to 21.3% at 18[o]C. The rescued males are fertile.

stck^{wt.T:Zzzz\FLAG} increases the frequency of male flw^G0172 escapers from 3.1% to 22.5% at 18[o]C. The rescued males are fertile. stck^{wt.T:Zzzz\FLAG} increases the small size of flw^G0172 larvae, such that they are increased in size compared to wild type. The reduced motility of flw^G0172 larvae is rescued by stck^{wt.T:Zzzz\FLAG}, with the rescued larvae being significantly more motile than wild type. The detachment of ventral intersegmental muscles seen in flw^G0172 larvae is partially rescued.

At 25[o]C, stck^{wt.T:Zzzz\FLAG} fully rescues the larval lethality of flw^G0172 mutants, but the pupal lethality is not rescued.

stck^{wt.T:Zzzz\FLAG} partially but significantly rescues the poor climbing ability of Mhc^S1 heterozygous adults. Hypercontraction of the indirect flight muscles is not rescued in these animals. Only 2% of the rescued animals have indentations in the dorsal thorax.

(Pronovost et al., 2013)

ics^BG02577 has no effect on the viability of flies expressing stck^{wt.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background.

(Elias et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

stck^wt.Tag:FLAG rescues stck¹⁸/stck^3R-17

(Elias et al., 2012)

Comments

Expression of stck^{wt.T:Zzzz\FLAG} rescues the embryonic and larval lethality seen in stck^3R-17/stck¹⁸ mutants. The rescued flies do not display any overt phenotypes.

(Elias et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66575

w^*; P{stck-FLAG.wt}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

stck^{wt.T:Zzzz\FLAG}

stck^wt.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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