FlyBase Allele Report: Dmel\flw[6]

General Information

Symbol

Dmel\flw⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0104623

Feature type

allele

Associated gene

Dmel\flw

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Raghavan et al., 2000)

Mutagen

ethyl methanesulfonate

(Raghavan et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: Y133F. Nucleotide substitution: TAC?TTC

(Raghavan et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:10,406,698..10,406,698 [+]

Nucleotide change:

A10406698T

Reported nucleotide change:

A?T

Amino acid change:

Y264F | flw-PA; Y133F | flw-PB; Y133F | flw-PC; Y133F | flw-PD

Reported amino acid change:

Y133F

(Raghavan et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape

(Wang and Riechmann, 2007)

abnormal locomotor behavior

(Raghavan et al., 2000)

flightless

(Raghavan et al., 2000)

lethal

(Pronovost et al., 2013)

lethal | male

(Vereshchagina et al., 2004)

lethal | recessive

(Kirchner et al., 2007)

partially lethal - majority die

(Kirchner et al., 2007, Raghavan et al., 2000)

visible

(Raghavan et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

flw⁶/Y flies have a survival rate of less than 1%.

(Kirchner et al., 2007)

flw⁶ homozygous mutant egg chambers generate an irregular wavy egg chamber epithelium. Mutant egg chambers are not round or ellipsoid like in wild-type but are either stretched or develop bulges.

(Wang and Riechmann, 2007)

On the rare occasion that a flw⁶ male fly survives to adulthood, it has severely crumpled wings. Egg chambers that are flw⁶ mutant within adult flies can be generated by expressing flw^Scer\UAS.cRa under the control of Scer\GAL4^arm.PS in somatic cells, so that somatic cells express wild-type flw but the female germline does not. The flw⁶ homozygous egg chambers have disrupted actin cytoskeletons and abnormal nuclear structures that indicate a failure of the transfer of nurse cell contents into the oocyte at stages 10B and 11. Additionally, the ring canals, which join nurse cells to each other and to the oocyte, fail to grow properly and are therefore smaller than in wild type.

(Vereshchagina et al., 2004)

flw¹/flw⁶ flies are completely flightless. Indirect flight muscles (IFMs) are never present in flw¹/flw⁶ flies, but the jump muscle (tergal depressor of trochanter muscle) appears normal. Less than 1% of flw⁶/Y males survive to adulthood at 25^oC. The period of lethality extends from the second larval instar onwards. Dying larvae are very sluggish. The muscle pattern in these larvae is clearly disrupted, many muscles are missing or weakly attached. In the few flw⁶/Y males that do eclose exhibit poor ambulatory ability, IFMs are absent and the tergal depressor of trochanter muscle (TDT) disorganised. Wings are crumpled and/or blistered.

(Raghavan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

flw⁶ has lethal phenotype, suppressible by stck^wt.Tag:FLAG

(Pronovost et al., 2013)

flw⁶ has lethal | recessive phenotype, suppressible by bsk¹/bsk[+]

(Kirchner et al., 2007)

flw⁶ has lethal | recessive phenotype, suppressible by bsk²

(Kirchner et al., 2007)

flw⁶ has lethal | male phenotype, suppressible by Rho1^E3.10

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by RhoGEF2⁰⁴²⁹¹

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by Tm1⁰²²⁹⁹

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by Tm1^Su(flw)4

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by sqh^A20.Tag:FLAG

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by sqh^A21.Tag:FLAG

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by zip⁰²⁹⁵⁷

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by zip^IIX62

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by zip^Su(flw)1

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by zip^Su(flw)2

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by zip^Su(flw)3

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by zip¹

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by sqh^{A20.A21.Tag:FLAG}

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, suppressible by Mbs^L.hs

(Vereshchagina et al., 2004)

NOT suppressed by

Statement

Reference

flw⁶ has partially lethal - majority die phenotype, non-suppressible by Pp1-87B^UAS.Tag:HA/Scer\GAL4^arm.PS

(Kirchner et al., 2007)

flw⁶ has partially lethal - majority die phenotype, non-suppressible by Scer\GAL4^arm.PS/Pp1α-96A^UAS.Tag:HA

(Kirchner et al., 2007)

flw⁶ has lethal | male phenotype, non-suppressible by MYPT-75D^{HM.UAS.Tag:MYC,}/Scer\GAL4^arm.PS

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, non-suppressible by MYPT-75D^{HM.UAS.Tag:MYC,}/Scer\GAL4^hs.PB

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, non-suppressible by Tm2^J8

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Vereshchagina et al., 2004)

flw⁶ has lethal | male phenotype, non-suppressible by sqh^E20.E21

(Vereshchagina et al., 2004)

Enhancer of

Statement

Reference

flw[+]/flw⁶ is an enhancer of decreased size | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

flw[+]/flw⁶ is an enhancer of visible | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

flw⁶/flw⁶ is an enhancer of majority die during embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

flw⁶ is an enhancer of majority die during embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

flw⁶ has wing phenotype, suppressible by Tm1^Su(flw)4

(Vereshchagina et al., 2004)

flw⁶ has wing phenotype, suppressible by zip^Su(flw)1

(Vereshchagina et al., 2004)

flw⁶ has wing phenotype, suppressible by zip^Su(flw)2

(Vereshchagina et al., 2004)

flw⁶ has wing phenotype, suppressible by zip^Su(flw)3

(Vereshchagina et al., 2004)

NOT suppressed by

Statement

Reference

flw⁶ has phenotype, non-suppressible by Mlc2^S66A,S67A

(Raghavan et al., 2000)

Enhancer of

Statement

Reference

flw[+]/flw⁶ is an enhancer of wing phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

flw⁶/flw⁶ is an enhancer of embryo | dorsal closure stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

flw⁶/flw⁶ is an enhancer of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

flw⁶ is an enhancer of embryo | dorsal closure stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

flw⁶ is an enhancer of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

flw⁶/flw⁶ or flw⁶ hemizygosity enhances the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background.

(Flores-Benitez and Knust, 2015)

stck^{wt.T:Zzzz\FLAG} increases the frequency of male flw⁶ escapers from 2.4% to 21.3% at 18[o]C. The rescued males are fertile.

(Pronovost et al., 2013)

The viability of flw⁶ mutants is much greater in a sqh^{A21.T:Zzzz\FLAG} background than a sqh^{A20.T:Zzzz\FLAG} background. The crumpled wing phenotype of flw⁶ flies is partially rescued by zip^Su(flw)1, zip^Su(flw)2 and zip^Su(flw)3 and fully rescued by Tm1^Su(flw)4.

(Vereshchagina et al., 2004)

The addition of Mlc2^S66A,S67A does not suppress flw mutant phenotypes.

(Raghavan et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

flw⁶ is rescued by flw^UAS.Tag:HA/Scer\GAL4^arm.PS

(Kirchner et al., 2007)

flw⁶ is rescued by Scer\GAL4^how-24B/Scer\GAL4^69B/flw^UAS.cRa

(Raghavan et al., 2000)

flw⁶ is rescued by flw^Hsp26.PR

(Raghavan et al., 2000)

Partially rescued by

flw⁶ is partially rescued by Scer\GAL4^arm.PS/flw^UAS.cRa

(Vereshchagina et al., 2004)

Not rescued by

flw⁶ is not rescued by Scer\GAL4^69B/flw^UAS.cRa

(Raghavan et al., 2000)

flw⁶ is not rescued by Scer\GAL4^how-24B/flw^UAS.cRa

(Raghavan et al., 2000)

Comments

Over 80% of males survive when flw^Scer\UAS.cRa is expressed, under the control of Scer\GAL4^arm.PS, in a flw⁶ background. The transgene also rescues the crumpled wing phenotype in surviving flw⁶ males.

(Vereshchagina et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

23693

w^* flw⁶/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PP1β9C⁶

(Kirchner et al., 2007)

flw⁶

(Hildebrand et al., 2021, Flores-Benitez and Knust, 2015, Pronovost et al., 2013, Yamamoto et al., 2013, Kirchner et al., 2007, Kirchner et al., 2007, Wang and Riechmann, 2007)

Name Synonyms

Secondary FlyBase IDs

References (9)

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