FlyBase Allele Report: Dmel\Eph[x652]

General Information

Symbol

Dmel\Eph^x652

Species

D. melanogaster

Name

FlyBase ID

FBal0196836

Feature type

allele

Associated gene

Dmel\Eph

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Boyle et al., 2006)

Mutagen

Delta2-3 transposase

(Boyle et al., 2006)

Progenitor genotype

P{hsp26-pt-T}P114

(Boyle et al., 2006)

Caused by aberration

Df(4)Eph^x652

Cytology

Description

Imprecise excision of the P{hsp26-pt-T} element that results in deletion of the first three exons of Eph (up to amino acid residue 79 of the expressed protein) and 5' sequences of the onecut gene (up to 368bp from its translation start site).

(Boyle et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | larval stage

(Howell et al., 2012)

abnormal neuroanatomy

(Boyle et al., 2006)

fertile | recessive

(Boyle et al., 2006)

viable | recessive

(Boyle et al., 2006)

Phenotype Manifest In

medial lobe of adult mushroom body

(Boyle et al., 2006)

vertical lobe of adult mushroom body

(Boyle et al., 2006)

vertical lobe of adult mushroom body | somatic clone

(Boyle et al., 2006)

Detailed Description

Statement

Reference

Mutant larvae fail to encapsulate eggs when parasitised by the avirulent L. boulardi wasp strain G486 (no melanisation is seen). Plasmatocytes fully cover the egg, while lamellocytes partially cover it.

(Howell et al., 2012)

The Eph^x652 mutation has only a minor effect on baseline synaptic transmission at the neuromuscular junction (NMJ).

(Frank et al., 2009)

Eph^x652 flies have no obvious morphological defects.

Eph^x652 embryos show no obvious defect in overall axon tract organization.

Eph^x652 mutants show defects in the formation of the dorsal lobe of the mushroom body throughout development. Both Eph^x652 embryos and pupae show reduced dorsal projections. In 60% of Eph^x652 third instar larvae, the dorsal lobe projections of the γ neurons are either reduced compared to wild type or missing. Reduced dorsal lobe projections are accompanied by increased medial axonal projections. In 60% of adults, the α' and α lobes are reduced or absent and the β' and β lobes show an increase in thickness. In 10% of cases the β lobes appear to be fused at the midline.

Analysis of individual Eph^x652 α/β neuron clones within the mushroom body shows that these neurons bifurcate normally, but sometimes extend both branches into the β lobe, instead of extending one branch dorsally along the α lobe. When this defect occurs, supernumerary branches can be seen along the length of the β lobe. The extent to which the neuron projections are defective reflects the overall severity of the mushroom body phenotype; in Eph^x652 mushroom bodies where the α lobe is missing, all neurons in a clone show projection defects, while in mushroom bodies with a normal overall morphology, α/β neuron clones show normal bifurcation and branching.

(Boyle et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Eph^x652 has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^ey-OK107/Ephrin^UAS.Tag:MYC

(Boyle et al., 2006)

Eph^x652 has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^elav.PLu/Ephrin^UAS.Tag:MYC

(Boyle et al., 2006)

NOT Enhancer of

Statement

Reference

Eph[+]/Eph^x652 is a non-enhancer of abnormal neurophysiology | third instar larval stage | recessive phenotype of GluRIIA^SP16

(Frank et al., 2009)

Suppressor of

Statement

Reference

Eph^x652 is a suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of GluRIIA^SP16

(Frank et al., 2009)

Exn^EY01953, Exn[+], Eph[+], Eph^x652 is a suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of GluRIIA^SP16

(Frank et al., 2009)

NOT Suppressor of

Statement

Reference

Eph[+]/Eph^x652 is a non-suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of GluRIIA^SP16

(Frank et al., 2009)

Other

Statement

Reference

Eph^x652, GluRIIA^SP16 has abnormal neurophysiology | recessive | third instar larval stage phenotype

(Frank et al., 2009)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Eph^x652 has mushroom body vertical lobe phenotype, non-suppressible by Scer\GAL4^ey-OK107/Ephrin^UAS.Tag:MYC

(Boyle et al., 2006)

Eph^x652 has mushroom body vertical lobe phenotype, non-suppressible by Scer\GAL4^elav.PLu/Ephrin^UAS.Tag:MYC

(Boyle et al., 2006)

NOT Enhancer of

Statement

Reference

Eph[+]/Eph^x652 is a non-enhancer of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Suppressor of

Statement

Reference

Exn^EY01953, Exn[+], Eph[+], Eph^x652 is a suppressor of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Eph^x652 is a suppressor of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Eph^x652 is a suppressor of symmetrical commissure phenotype of Ephrin^UAS.Tag:MYC, Scer\GAL4^sim.PS

(Boyle et al., 2006)

NOT Suppressor of

Statement

Reference

Eph[+]/Eph^x652 is a non-suppressor of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Eph^x652 is a non-suppressor of mushroom body vertical lobe phenotype of Ephrin^UAS.Tag:MYC, Scer\GAL4^elav.PLu

(Boyle et al., 2006)

Eph^x652 is a non-suppressor of mushroom body vertical lobe phenotype of Ephrin^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Boyle et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The neuromuscular junction (NMJ) bouton numbers in GluRIIA^SP16; ;Eph^x652 double mutants are not significantly different from that of the GluRIIA^SP16 single mutant.

The spontaneous miniature release event (mepsp) amplitudes are significantly decreased in GluRIIA^SP16; ;Eph^x652 double mutants compared to Eph^x652 single mutants or wild-type. This double mutant phenotype is similar to the one observed in GluRIIA^SP16 single mutants.

The average homeostatic increase in presynaptic release observed in GluRIIA^SP16 single mutants is significantly suppressed in GluRIIA^SP16; ;Eph^x652 double mutants.

The heterozygous Eph^x652/+ mutation alone does not significantly change the recessive GluRIIA^SP16 phenotype involving the synaptic homeostatic compensation at the neuromuscular junction (NMJ).

The combination of Exn^EY01953/+ and Eph^x652/+ heterozygous mutations does not significantly change the GluRIIA^SP16 phenotype involving spontaneous miniature release event (mepsp) amplitudes, while it does partially suppress the GluRIIA^SP16 quantal content phenotype at the neuromuscular junction (NMJ).

(Frank et al., 2009)

Expression of Ephrin^{Scer\UAS.T:Hsap\MYC} in the midline glia of embryos, driven by Scer\GAL4^sim.PS, in an Eph^x652 background does not cause the disruption of commissural axons seen when the transgene is expressed in wild-type embryos.

Eph^x652/onecut^x122 and Eph^x652/onecut^x49 mutants show no defects in dorsal lobe formation, indicating that the dorsal lobe phenotype of Eph^x652 mutants is due to the disruption of Eph.

Flies that express Ephrin^{Scer\UAS.T:Hsap\MYC} under the control of either Scer\GAL4^OK107 or Scer\GAL4^elav.PLu, in an Eph^x652 background, show the same α'/α lobe defects as flies expressing the transgene in a wild-type background and as Eph^x652 flies with no transgene.

(Boyle et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Eph^X652

(Anzo et al., 2017, Singh et al., 2013)

Eph^x652

(Howell et al., 2012, Frank et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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