FlyBase Allele Report: Dmel\Tctp[RNAi.UAS]

General Information

Symbol

Dmel\Tctp^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0197205

Feature type

allele

Associated gene

Dmel\Tctp

Associated Insertion(s)

Carried in Construct

P{UAS-Tctp.RNAi}

Also Known As

UAS-Tctp-i, UAS-Tctp-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Hsu et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Hsu et al., 2007)

Mutagen

in vitro construct

(Hsu et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Tctp.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 389bp fragment of Tctp.

(Hsu et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tctp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain cancer

modeled by l(3)mbt^ts1

(Rossi et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell growth rate | third instar larval stage, with Scer\GAL4^AB1

(Nam et al., 2022)

decreased cell number, with Scer\GAL4^nub.PK

(Hsu et al., 2007)

decreased cell size, with Scer\GAL4^nub.PK

(Hsu et al., 2007)

decreased cell size | third instar larval stage, with Scer\GAL4^AB1

(Nam et al., 2022)

decreased size | adult stage, with Scer\GAL4^ey.PH

(Nam et al., 2022)

decreased size | adult stage, with Scer\GAL4^nub.PU

(Yang et al., 2021)

decreased size | pharate adult stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

decreased size | third instar larval stage, with Scer\GAL4^AB1

(Nam et al., 2022)

decreased size | third instar larval stage, with Scer\GAL4^en.PU

(Yang et al., 2021)

decreased size | third instar larval stage, with Scer\GAL4^ey.PH

(Nam et al., 2022)

increased cell number | third instar larval stage, with Scer\GAL4^AB1

(Nam et al., 2022)

lethal - all die before end of larval stage, with Scer\GAL4^Act.PU

(Hsu et al., 2007)

some die during third instar larval stage, with Scer\GAL4^Act.PU

(Hsu et al., 2007)

visible, with Scer\GAL4^ey.PH

(Hong and Choi, 2013, Hsu et al., 2007)

visible, with Scer\GAL4^nub.PK

(Hsu et al., 2007)

visible, with Scer\GAL4^ptc-559.1

(Hsu et al., 2007)

visible | adult stage, with Scer\GAL4^ey.PH

(Nam et al., 2022)

Phenotype Manifest In

1st posterior cell, with Scer\GAL4^ptc-559.1

(Hsu et al., 2007)

embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4^AB1

(Nam et al., 2022)

eye, with Scer\GAL4^ey.PH

(Nam et al., 2022, Hong and Choi, 2013, Hsu et al., 2007)

eye | adult stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

eye disc posterior to the morphogenetic furrow, with Scer\GAL4^ey.PH

(Nam et al., 2022)

wing, with Scer\GAL4^nub.PK

(Hsu et al., 2007)

wing, with Scer\GAL4^nub.PU

(Yang et al., 2021)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en.PU

(Yang et al., 2021)

Detailed Description

Statement

Reference

The expression of Tctp^dsRNA.UAS under the control of Scer\GAL4^ey.PH induces a small eye phenotype; eye discs are small and show fewer columns of photoreceptor clusters than controls.

The expression of Tctp^dsRNA.UAS under the control of Scer\GAL4^AB1 induces a small third instar larval salivary gland, which presents more but smaller cells, with decreased cell growth (decreased endoreplication).

(Nam et al., 2022)

The expression of Tctp^dsRNA.UAS under the control of Scer\GAL4^ey.PU induces a mild small eye phenotype. Expression under the control of Scer\GAL4^nub.PU induces a small wing, which can also be severely wrinkled.

The expression of Tctp^dsRNA.UAS under the control of Scer\GAL4^en.PU induces a small third instar larval wing disc posterior compartment.

(Yang et al., 2021)

G2/M arrest after gamma irradiation is compromised in the posterior compartment of the wing disc in animals expressing Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en.PU.

Expression of Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PH results in a small eye phenotype.

(Hong and Choi, 2013)

Expression of Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PH results in a reduction in eye size.

Expression of Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^nub.PK results in a reduction in the size of the wing. This reduction in size is due to a decrease in both cell size and cell number.

Expression of Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 reduces the distance between veins L3 and L4 in the wing.

Expression of Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act results in lethality around the third larval instar. Some of these larvae are able to survive to the pupal stage with reduced body size.

(Hsu et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has decreased size | adult stage phenotype, enhanceable by foxo^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^ey.PH

(Nam et al., 2022)

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has visible | adult stage phenotype, enhanceable by foxo^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^ey.PH

(Nam et al., 2022)

Scer\GAL4^ey.PU, Tctp^RNAi.UAS has decreased size | adult stage phenotype, enhanceable by Top2^suo1/Top2[+]

(Yang et al., 2021)

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has visible phenotype, enhanceable by Df(3R)PG4/+, Scer\GAL4^ey.PH

(Hong and Choi, 2013)

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has visible phenotype, enhanceable by tefu[+]/tefu^atm-6/Scer\GAL4^ey.PH

(Hong and Choi, 2013)

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has visible phenotype, enhanceable by S6k[+]/S6k^l-1

(Hsu et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^nub.PU, Tctp^RNAi.UAS has decreased size | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/Top2^UAS.cYa

(Yang et al., 2021)

Scer\GAL4^ptc-559.1, Tctp^RNAi.UAS has visible phenotype, suppressible by Scer\GAL4^ey.PH/Hsap\TPT1^UAS.cHa

(Hsu et al., 2007)

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has visible phenotype, suppressible by Hsap\TPT1^UAS.cHa, Scer\GAL4^ey.PH

(Hsu et al., 2007)

Enhancer of

Statement

Reference

Tctp^RNAi.UAS/Scer\GAL4^ey.PH is an enhancer of decreased size | adult stage phenotype of Scer\GAL4^ey.PH, foxo^{UAS.ORF.GW.Tag:HA}

(Nam et al., 2022)

Tctp^RNAi.UAS/Scer\GAL4^ey.PH is an enhancer of visible | adult stage phenotype of Scer\GAL4^ey.PH, foxo^{UAS.ORF.GW.Tag:HA}

(Nam et al., 2022)

Tctp^RNAi.UAS/Scer\GAL4^ey.PU is an enhancer of decreased size | adult stage | dominant phenotype of Top2^suo1

(Yang et al., 2021)

Suppressor of

Statement

Reference

Tctp^RNAi.UAS/Scer\GAL4^ey.PH is a suppressor of visible | somatic clone phenotype of Tsc1^Q600X

(Hsu et al., 2007)

Other

Statement

Reference

Scer\GAL4^ey.PU, Tctp^RNAi.UAS, Top2^JF01300 has lethal - all die before end of P-stage phenotype

(Yang et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has eye phenotype, enhanceable by foxo^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^ey.PH

(Nam et al., 2022)

Scer\GAL4^nub.PU, Tctp^RNAi.UAS has wing phenotype, enhanceable by Scer\GAL4^nub.PU/Top2^JF01300

(Yang et al., 2021)

Scer\GAL4^ey.PU, Tctp^RNAi.UAS has eye | female phenotype, enhanceable by Top2^suo1/Top2[+]

(Yang et al., 2021)

Scer\GAL4^ey.PU, Tctp^RNAi.UAS has wing phenotype, enhanceable by Top2^suo1/Top2[+]

(Yang et al., 2021)

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has eye phenotype, enhanceable by Df(3R)PG4/+, Scer\GAL4^ey.PH

(Hong and Choi, 2013)

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has eye phenotype, enhanceable by tefu[+]/tefu^atm-6/Scer\GAL4^ey.PH

(Hong and Choi, 2013)

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has eye phenotype, enhanceable by S6k[+]/S6k^l-1

(Hsu et al., 2007)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ey.PU, Tctp^RNAi.UAS has eye | male phenotype, non-enhanceable by Top2^suo1/Top2[+]

(Yang et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^nub.PU, Tctp^RNAi.UAS has wing phenotype, suppressible | partially by Scer\GAL4^nub.PU/Top2^UAS.cYa

(Yang et al., 2021)

Scer\GAL4^ptc-559.1, Tctp^RNAi.UAS has 1st posterior cell phenotype, suppressible by Scer\GAL4^ey.PH/Hsap\TPT1^UAS.cHa

(Hsu et al., 2007)

Scer\GAL4^ey.PH, Tctp^RNAi.UAS has eye phenotype, suppressible by Hsap\TPT1^UAS.cHa, Scer\GAL4^ey.PH

(Hsu et al., 2007)

Enhancer of

Statement

Reference

Tctp^RNAi.UAS/Scer\GAL4^ey.PH is an enhancer of eye phenotype of Scer\GAL4^ey.PH, foxo^{UAS.ORF.GW.Tag:HA}

(Nam et al., 2022)

Scer\GAL4^nub.PU/Tctp^RNAi.UAS is an enhancer of wing phenotype of Scer\GAL4^nub.PU, Top2^JF01300

(Yang et al., 2021)

Tctp^RNAi.UAS/Scer\GAL4^ey.PU is an enhancer of eye phenotype of Top2^suo1

(Yang et al., 2021)

Suppressor of

Statement

Reference

Tctp^RNAi.UAS/Scer\GAL4^ey.PH is a suppressor of eye | somatic clone phenotype of Tsc1^Q600X

(Hsu et al., 2007)

Tctp^RNAi.UAS/Scer\GAL4^ey.PH is a suppressor of adult head | somatic clone phenotype of Tsc1^Q600X

(Hsu et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The small eye phenotype caused by expression of Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PH is enhanced if the flies are heterozygous for either Df(3R)PG4 or tefu^atm-6.

(Hong and Choi, 2013)

The distance between wing veins L3 and L4 is smaller than normal in flies co-expressing InR^Scer\UAS.cHa and Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1, resembling the phenotype seen in flies expressing Tctp^{dsRNA.Scer\UAS} alone under the control of Scer\GAL4^ptc-559.1.

The distance between wing veins L3 and L4 is smaller than normal in flies co-expressing Rheb^Scer\UAS.cSa and Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1, resembling the phenotype seen in flies expressing Tctp^{dsRNA.Scer\UAS} alone under the control of Scer\GAL4^ptc-559.1.

The reduction in eye size seen in animals expressing Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PH is enhanced if they are also carrying S6k^l-1/+.

(Hsu et al., 2007)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\TPT1^Scer\UAS.cHa suppresses the reduction in eye size seen in animals expressing Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PH.

Co-expression of Hsap\TPT1^Scer\UAS.cHa suppresses the phenotype of a reduced distance between wing veins L3 and L4 which is seen in animals expressing Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1.

(Hsu et al., 2007)

Complementation and Rescue Data

Rescued by

Tctp^RNAi.UAS is rescued by Tctp^UAS.cHa/Scer\GAL4^ey.PH

(Hsu et al., 2007)

Partially rescued by

Tctp^RNAi.UAS is partially rescued by Tctp^UAS.cHa/Scer\GAL4^AB1

(Nam et al., 2022)

Not rescued by

Tctp^RNAi.UAS is not rescued by Tctp^E12V.UAS/Scer\GAL4^ey.PH

(Hsu et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

dsRNA has been synthesised from the template and used to study the phenotypic consequences of dsRNA interference (RNAi) of the Tctp gene.

(Hsu et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tctp^RNAi.UAS

Tctp^{dsRNA.Scer\UAS}

Tctp^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (9)

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