FlyBase Allele Report: Dmel\Rala[EE1]

General Information

Symbol

Dmel\Rala^EE1

Species

D. melanogaster

Name

FlyBase ID

FBal0197295

Feature type

allele

Associated gene

Dmel\Rala

Associated Insertion(s)

Carried in Construct

Also Known As

Ral^EE1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Eun et al., 2007)

Progenitor genotype

Cytology

Description

Amino acid replacement: S154L.

(Eun et al., 2007)

Nucleotide substitution: C?T.

(Eun et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:3,708,772..3,708,772 [-]

Nucleotide change:

C3708772T

Reported nucleotide change:

C?T

Amino acid change:

S154L | Rala-PA; S154L | Rala-PB; S154L | Rala-PC

Reported amino acid change:

S154L

(Eun et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight | male

(Richhariya et al., 2017)

abnormal planar polarity

(Cho and Fischer, 2011)

abnormal planar polarity | somatic clone

(Cho and Fischer, 2011)

male sterile

(Eun et al., 2007)

viable

(Eun et al., 2007)

visible

(Cho and Fischer, 2011, Eun et al., 2007)

Phenotype Manifest In

adult head bristle

(Eun et al., 2007)

chaeta & mesothoracic tergum

(Eun et al., 2007)

eye

(Eun et al., 2007)

eye photoreceptor cell

(Cho and Fischer, 2011)

humeral bristle

(Eun et al., 2007)

ommatidial precursor cluster

(Cho and Fischer, 2011)

ommatidium

(Cho and Fischer, 2011)

ommatidium | somatic clone

(Cho and Fischer, 2011)

wing blade

(Eun et al., 2007)

Detailed Description

Statement

Reference

Rala^EE1 hemizygous males are almost completely flightless but heterozygous females show normal flight ability.

(Richhariya et al., 2017)

Ommatidia in adult mutant males have a number of defects, including loss of photoreceptor cells, loss of R3/R4 asymmetry and defects in orientation with respect to the eye equator. In mutant third instar eye discs, many ommatidia rotate either two much or too little and R1 and R6 are frequently absent.

In mosaic ommatidia where both of the R3/R4 pair are Rala[+], the ommatidia are asymmetrical and have normal chirality. In mosaic ommatidia where both of the R3/R4 pair are homozygous, approximately 10% of the ommatidia are symmetrical. In ommatidia where one of the R3/R4 pair is Rala[+] and the other is homozygous, different results are seen depending on which of the R3/R4 precursors is Rala[+]; when the polar cell (pre-R4) is homozygous, all of the ommatidia are wild type, but when the equatorial cell (pre-R3) is homozygous, approximately 7% of the ommatidia are symmetrical and 2% have reversed chirality.

(Cho and Fischer, 2011)

Hemizygous males have rough eyes and bristles on the head and humerus are either missing or severely truncated. The notal bristles and hairs are missing. The wings are short and curved.

(Eun et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rala^EE1 has visible phenotype, enhanceable by Delta^9P/Dl[+]

(Cho and Fischer, 2011)

Rala^EE1 has visible phenotype, enhanceable by neur[+]/neur¹

(Cho and Fischer, 2011)

Rala^EE1 has visible phenotype, enhanceable | heat sensitive by N^GV.3.hs

(Cho and Fischer, 2011)

Rala^EE1 has visible phenotype, enhanceable | heat sensitive by N::sev^{Sev11.GV.3.hs}

(Cho and Fischer, 2011)

Suppressed by

Statement

Reference

Rala^EE1 has abnormal planar polarity phenotype, suppressible by lqf^AG/lqf[+]

(Cho and Fischer, 2011)

Rala^EE1 has visible phenotype, suppressible by lqf^AG/lqf[+]

(Cho and Fischer, 2011)

Enhancer of

Statement

Reference

Rala[+]/Rala^EE1 is an enhancer of visible phenotype of lqf^{glrs.Tag:FLAG}

(Eun et al., 2007)

Suppressor of

Statement

Reference

Rala[+]/Rala^EE1 is a suppressor of visible phenotype of lqf^FDD9

(Cho and Fischer, 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rala^EE1 has eye phenotype, enhanceable by Delta^9P/Dl[+]

(Cho and Fischer, 2011)

Rala^EE1 has eye phenotype, enhanceable by neur[+]/neur¹

(Cho and Fischer, 2011)

Rala^EE1 has eye phenotype, enhanceable | heat sensitive by N^GV.3.hs

(Cho and Fischer, 2011)

Rala^EE1 has eye phenotype, enhanceable | heat sensitive by N::sev^{Sev11.GV.3.hs}

(Cho and Fischer, 2011)

Suppressed by

Statement

Reference

Rala^EE1 has ommatidium phenotype, suppressible by lqf^AG/lqf[+]

(Cho and Fischer, 2011)

Rala^EE1 has eye photoreceptor cell phenotype, suppressible by lqf^AG/lqf[+]

(Cho and Fischer, 2011)

Enhancer of

Statement

Reference

Rala[+]/Rala^EE1 is an enhancer of eye phenotype of lqf^{glrs.Tag:FLAG}

(Eun et al., 2007)

Suppressor of

Statement

Reference

Rala[+]/Rala^EE1 is a suppressor of ommatidium phenotype of lqf^FDD9

(Cho and Fischer, 2011)

Rala[+]/Rala^EE1 is a suppressor of eye photoreceptor cell phenotype of lqf^FDD9

(Cho and Fischer, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Rala^EE1 dominantly suppresses the rough eye phenotype of lqf^FDD9 homozygotes, with a greater proportion of ommatidia containing the normal number of photoreceptor cells in the double mutant flies.

lqf^AG dominantly suppresses the rough eye phenotype of Rala^EE1/Y adults, with a greater proportion of ommatidia being completely normal in the double mutant flies.

The rough eye phenotype of Rala^EE1/Y adults is dominantly enhanced by Dl^9P and by neur¹.

Expression of either N^{3.hs.T:Scer\GAL4,T:Hsim\VP16} or N::sev^{Sev11.3.hs.T:Scer\GAL4,T:Hsim\VP16} using heat shock (either 1 or 2 hours) during the third instar larval stage enhances the rough eye phenotype of Rala^EE1/Y.

(Cho and Fischer, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rala^EE1 is rescued by Scer\GAL4^Act5C.PI/Rala^UAS.cSa

(Eun et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25095

Rala^EE1/FM7c

Notes on Origin

Discoverer

Selected as: A dominant enhancer of the lqf^{glrs.T:Zzzz\FLAG} rough eye phenotype.

(Eun et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

EE1

(Eun et al., 2007)

Ral^EE1

(Richhariya et al., 2017, Cho and Fischer, 2011)

Rala^EE1

ralA^EE1

(Koon et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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