FlyBase Allele Report: Dmel\Uba1[A1]

General Information

Symbol

Dmel\Uba1^A1

Species

D. melanogaster

Name

FlyBase ID

FBal0197410

Feature type

allele

Associated gene

Dmel\Uba1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Pfleger et al., 2007)

Progenitor genotype

Cytology

Description

Internal deletion of 235bp of coding sequence, resulting in a frameshift at amino acid residue 545, which truncates the protein prior to the second THIF domain and results in the addition of 25 novel amino acid residues.

(Pfleger et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:9,690,596..9,690,830 [-]

Comment:

Deletion of 235bp, which results in a frameshift after amino acid residue 545 and adds 25 novel amino acid residues. The position of the deletion was unspecified and is approximate. Estimated to be within +/- 2 bases of actual position.

(Pfleger et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | cell non-autonomous | somatic clone

(Yan et al., 2009)

short lived | dominant

(Liu and Pfleger, 2013)

visible | somatic clone

(Pfleger et al., 2007)

Phenotype Manifest In

eye | cell non-autonomous | somatic clone

(Pfleger et al., 2007)

eye disc | cell non-autonomous | somatic clone

(Yan et al., 2009)

Detailed Description

Statement

Reference

Uba1^A1/+ mutant flies display a significant decrease in lifespan as compared to wild type.

(Liu and Pfleger, 2013)

Eyes containing Uba1^A1 homozygous somatic clones exhibit a non-autonomous overgrowth phenotype.

(Yan et al., 2009)

Homozygous clones in the eye result in overgrowth of surrounding wild-type tissue.

(Pfleger et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Uba1^A1 has visible | somatic clone phenotype, enhanceable by Df(3L)H99/+

(Pfleger et al., 2007)

Suppressed by

Statement

Reference

Uba1^A1 has increased cell number | somatic clone | cell non-autonomous phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Yan et al., 2009)

Uba1^A1 has visible | somatic clone phenotype, suppressible by th[+]/Diap1^6B

(Pfleger et al., 2007)

NOT suppressed by

Statement

Reference

Uba1^A1 has increased cell number | somatic clone | cell non-autonomous phenotype, non-suppressible by Akt1[+]/Akt⁰⁴²²⁶

(Yan et al., 2009)

Enhancer of

Statement

Reference

Uba1^A1 is an enhancer of visible | somatic clone phenotype of hpo^MGH1

(Pfleger et al., 2007)

Other

Statement

Reference

PTP-ER^f02707, Uba1^A1 has increased cell number | somatic clone | cell non-autonomous phenotype

(Yan et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Uba1^A1 has eye | somatic clone phenotype, enhanceable by Df(3L)H99/+

(Pfleger et al., 2007)

Suppressed by

Statement

Reference

Uba1^A1 has eye disc | somatic clone | cell non-autonomous phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Yan et al., 2009)

Uba1^A1 has eye | somatic clone phenotype, suppressible by th[+]/Diap1^6B

(Pfleger et al., 2007)

NOT suppressed by

Statement

Reference

Uba1^A1 has eye disc | somatic clone | cell non-autonomous phenotype, non-suppressible by Akt1[+]/Akt⁰⁴²²⁶

(Yan et al., 2009)

Enhancer of

Statement

Reference

Uba1^A1 is an enhancer of eye | somatic clone phenotype of hpo^MGH1

(Pfleger et al., 2007)

Other

Statement

Reference

PTP-ER^f02707, Uba1^A1 has eye disc | somatic clone | cell non-autonomous phenotype

(Yan et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

A Ras85D^e1B/+ background dramatically suppresses the non-autonomous overgrowth phenotype found in Uba1^A1 mosaic eye clones.

A Akt1⁰⁴²²⁶/+ background has no effect on the non-autonomous overgrowth phenotype found in Uba1^A1 mosaic eye clones.

Mosaic eyes in which cells homozygous for Uba1^A1 signal to cells homozygous for the mutation PTP-ER^f02707 resulting in a dramatic overgrowth phenotype where they also exhibit additional outgrowths in the PTP-ER^f02707 mutant tissue compared to mosaic eyes in which Uba1^A1 cells signal to wild-type cells. These outgrowths contain cuticle-like tissue as well as recognizable ommatidia with red pigment.

(Yan et al., 2009)

The overgrowth phenotype that is caused by homozygous clones in the eye is enhanced by Df(3L)H99/+ and is suppressed by th^6B/+.

Homozygous hpo^MGH1 clones in the eye result in an increase in the overall size of the eye. When the twin-spot is composed of Uba1^A1 tissue, the overgrowth is enhanced.

(Pfleger et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Uba1⁰³⁴⁰⁵

(Pfleger et al., 2007)

Uba1^s3484

(Pfleger et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Uba1^A1

(Liu and Pfleger, 2013, Yan et al., 2009, Pfleger et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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