Amino acid replacement: Q504term.
C19495185T
C?T
Q503term | hpo-PA; Q503term | hpo-PB
Q504term
A glutamine to stop mutation occuring in a polyglutamine stretch that is of varying lengths in different strains.
Homozygous clones generated using the eyFLP system result in eyes with an increased number of interommatidial cells compared to wild type.
Homozygous clones in the eye result in an increase in the overall size of the eye.
Eyes containing marked hpoMGH1 mutant and wild-type clones (generated throughout the eye disc using Scer\FLP1ey.PN with P{neoFRT}42D) have an over-representation of mutant tissue, suggesting that the mutant tissue has a relative growth advantage. Mutant ommatidial facets are slightly larger than wild-type facets and sometimes contain extra interommatidial bristles. The number of cells in hpoMGH1 mutant clones is consistently larger than in wild-type sister clones (clones generated at 48 hr after egg deposition (AED) and analyzed 72 hr later): The median population doubling time in these clones is 13.1 hr, which is significantly shorter than that for clone carrying a Avic\GFP bearing tester chromosome, which was 14.7 hr (P < 0.0001 by a Student's paired t test). Cell size in mutant clones is normal. Retinal sections reveal that ommatidia in hpoMGH1 mutant clones have the normal complement and arrangement of photoreceptor cells but are separated by significantly more interommatidial tissue. Additional layers interommatidial cells are apparent between ommatidia in mutant pupal retinas at 48 hours after puparium formation. At 38 hours APF, when excess interommatidial cells are eliminated by apoptosis, most of the cell death in discs containing hpoMGH1 mutant clones is restricted to wild-type portions of the disc. Homozygous clones of hpoMGH1 mutant cells in various imaginal discs, cause outgrowths of tissue. Portions of wings containing large hpoMGH1 clones are larger than the corresponding portion of a wild-type wing. In hpoMGH1 mosaic eye discs, the pattern of S phases is normal in the anterior portion of the disc and in the second mitotic wave (SMW) but in mutant portions these eye discs, S phase (BrdU-incorporating) nuclei can be seen posterior to the (SMW) and in the morphogenetic furrow (MF), a situation not seen in wild-type. i.e.- hpoMGH3 cells continue to replicate their DNA when surrounding wild-type cells are arrested in G1. Mitotic cells occur in mutant clones many ommatidial rows posterior to the SMW, a region devoid of mitoses in wild-type eye discs.
hpoMGH1 has visible | somatic clone phenotype, enhanceable by Uba1A1
hpoMGH1 is a suppressor of increased cell death | larval stage phenotype of grimGMR.PC
hpoMGH1 has interommatidial cell | increased number | somatic clone phenotype, enhanceable by RtGEFp1036
hpoMGH1 has interommatidial cell | increased number | somatic clone phenotype, enhanceable by Gitex21C
hpoMGH1 has eye | somatic clone phenotype, enhanceable by Uba1A1
hpoMGH1 is a suppressor of eye phenotype of grimGMR.PC
hpoMGH1 is a suppressor of ommatidium phenotype of grimGMR.PC
The increase in the number of interommatidial cells which is seen in homozygous hpoMGH1 clones generated using the eyFLP system is enhanced if the clones are also homozygous for either RtGEFp1036 or Gitex21C. No further enhancement over either double mutant phenotype is seen in the hpoMGH1, RtGEFp1036, Gitex21C triple mutant combination.
The small, rough eye phenotye due to savGMR.PT; wtsGMR.PT, is suppressed when somatic clones of hpoMGH1 homozygous cells are generated throughout the eye discs using Scer\FLP1ey.PN with P{neoFRT}42D. The small, rough eye phenotype of grimGMR.PC flies is suppressed when somatic clones of hpoMGH1 homozygous cells are generated throughout the eye discs using Scer\FLP1ey.PN with P{neoFRT}42D. In the eye discs from these animals, the abnormal levels of cell death posterior to the morphogenetic furrow seen in grimGMR.PC eye discs are suppressed in the hpoMGH1 mutant tissue.
