FlyBase Allele Report: Dmel\Git[ex21C]

General Information

Symbol

Dmel\Git^ex21C

Species

D. melanogaster

Name

FlyBase ID

FBal0221208

Feature type

allele

Associated gene

Dmel\Git

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Bahri et al., 2009)

Progenitor genotype

P{EPgy2}EY09254

(Bahri et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion of 857bp which extends from the original insertion site into the Git open reading frame, deleting sequences encoding the N-terminal 109 amino acid residues.

(Bahri et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage

(Hong and Mah, 2015)

abnormal size | adult stage

(Hong and Mah, 2015)

partially lethal - majority die | recessive

(Bahri et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Git^ex21C mutant adults exhibit severely reduced brain size as compared to controls, and a subset exhibit defects in mushroom body structure, with terminated alpha or beta lobes, or divided alpha lobes.

(Hong and Mah, 2015)

100% of homozygous escapers have defective wing morphology; the wings are crumpled, uneven and sometimes curled upwards or not fully spread. Git^ex21C/Git^ex51C adults also have a defective wing phenotype.

Homozygous escapers produce fewer fertilised eggs than normal.

Embryos lacking both maternal and zygotic Git[+] function show guidance defects in the VO5 and VO6 muscles, which are characterised by bypass and mistargeting of the mutant muscles towards the ventral midline; VO5 and VO6 muscles from opposite sides of late stage mutant embryos inappropriately attach at the ventral midline or fuse together at their meeting point, instead of terminating at a distance from the midline as occurs in wild-type embryos. 60% of VO5/VO6 muscles are affected to various degrees. The VA3 muscle occasionally shows mistargeting towards the ventral midline. There is a low level of increase in the number of lateral transverse (18% of hemisegments and VA3 (7.5%) muscles in these embryos, and in addition, there are low frequencies of muscle shape or attachment defects in the VO1 and LO1 muscles.

Embryos lacking zygotic Git[+] function show a moderate increase in muscle numbers compared to controls for LT (7.5%) and VA3 (3.7%) muscles and they also show defects in VA3 shape and targeting.

(Bahri et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Git^ex21C, ft⁸ has visible | somatic clone phenotype

(Dent et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Git^ex21C is an enhancer of interommatidial cell | increased number | somatic clone phenotype of hpo^MGH1

(Dent et al., 2015)

NOT Enhancer of

Statement

Reference

Git^ex21C is a non-enhancer of interommatidial cell | increased number | somatic clone phenotype of hpo^5.1

(Dent et al., 2015)

Other

Statement

Reference

Git^ex21C, ft⁸ has ptilinum | somatic clone phenotype

(Dent et al., 2015)

Git^ex21C, Pak²⁰ has presumptive embryonic/larval muscle system phenotype

(Bahri et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Git^ex21C mutants carrying ft⁸ clones generated using the eyFLP system show substantial overgrowth of the head, particularly in the ptilinum.

The increase in the number of interommatidial cells which is seen in homozygous hpo^MGH1 clones generated using the eyFLP system is enhanced if the clones are also homozygous for Git^ex21C.

The increase in the number of interommatidial cells which is seen in homozygous hpo^5.1 clones generated using the eyFLP system is not further enhanced if the clones are also homozygous for Git^ex21C.

(Dent et al., 2015)

Git^ex21C Pak²⁰ zygotic double mutant embryos show muscle targeting defects that are not seen in either zygotic mutant alone, and the double mutant embryos show a general disorganisation of their muscle pattern.

(Bahri et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Git^ex21C is partially rescued by Git^UAS.EGFP/Scer\GAL4^how-24B

(Bahri et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Git^ex21C

Git^ex21c

(Ho and Treisman, 2020)

dGit1^ex21C

(Hong and Mah, 2015)

dgit^21C

(Bahri et al., 2009)

dgit^ex21C

(Bahri et al., 2009)

git^ex21C

(Dent et al., 2019)

git^ex21c

(Dent et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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