FlyBase Allele Report: Dmel\Set2[RNAi.UAS]

General Information

Symbol

Dmel\Set2^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0197581

Feature type

allele

Associated gene

Dmel\Set2

Associated Insertion(s)

Carried in Construct

P{UAS-Set2.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Stabell et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Stabell et al., 2007)

Mutagen

in vitro construct

(Stabell et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Set2.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a 540bp inverted repeat corresponding to a region of Set2 cDNA. The Set2 sequences are separated by an H intron.

(Stabell et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Set2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Song et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage, with Scer\GAL4^da.G32

(Stabell et al., 2007)

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

(Stabell et al., 2007)

some die during third instar larval stage, with Scer\GAL4^da.G32

(Stabell et al., 2007)

visible, with Scer\GAL4^ap-md544

(Stabell et al., 2007)

Phenotype Manifest In

mitotic spindle | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Brockett et al., 2024)

wing, with Scer\GAL4^ap-md544

(Stabell et al., 2007)

Detailed Description

Statement

Reference

Animals that express Set2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 develop normally until the end of the third larval instar. However, these larvae fail to form their puparium and crawling larvae are still found after 10-12 days. The larvae finally stop moving, and in a few cases melanin-less "pseudo-prepupae" are formed, which maintain the elongated larval form and fail to evert the anterior spiracles. Feeding these larvae with 20-hydroxyecdysone (20E) does not rescue them to puparium formation.

Expression of Set2^{dsRNA.Scer\UAS} in the wing discs, driven by Scer\GAL4^ap-md544, generates a blister in the proximal part of the adult wing. The transparent blisters appear initially as large balloons filled with air and hemolymph and deflate deflate after a while, leaving a scar that may cause the wing to bend up. This phenotype shows 100% penetrance.

(Stabell et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, Set2^RNAi.UAS has visible phenotype, enhanceable by EcR^M554fs/EcR[+]

(Stabell et al., 2007)

Other

Statement

Reference

Scer\GAL4^fkh.PH, Set2^RNAi.UAS, p53^{UAS.A.Tag:MYC} has increased cell death phenotype

(Zhang et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, Set2^RNAi.UAS has wing phenotype, enhanceable by EcR^M554fs/EcR[+]

(Stabell et al., 2007)

Other

Statement

Reference

Scer\GAL4^fkh.PH, Set2^RNAi.UAS, p53^{UAS.A.Tag:MYC} has salivary gland phenotype

(Zhang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Set2^{dsRNA.Scer\UAS} and p53^{Scer\UAS.A.T:Hsap\MYC} under the control of Scer\GAL4^fkh.PH results in small salivary glands and high levels of apoptotic cell death.

(Zhang et al., 2014)

Expression of Set2^{dsRNA.Scer\UAS} in the wing discs of EcR^M554fs/+ flies, driven by Scer\GAL4^ap-md544, causes an enhanced wing phenotype compared to Set2^{dsRNA.Scer\UAS} expression in a wild-type background. The wing layers of Set2^{dsRNA.Scer\UAS}; Scer\GAL4^ap-md544; EcR^M554fs/+ flies are completely separated and the resulting balloon becomes blackened and remains inflated for 1-2 days. Often both wings are held in a "Dichaete-like" fashion.

(Stabell et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24108

y¹ w¹¹¹⁸; P{UAS-Set2.RNAi}21

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-Set2.IR} construct should produce Set2 dsRNA, resulting in dsRNA interference (RNAi) of the Set2 gene.

(Stabell et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Set2^RNAi.UAS

Set2^RNAi

(Brockett et al., 2024)

Set2^{dsRNA.Scer\UAS}

Set2^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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