FlyBase Allele Report: Dmel\Rack1[1.8]

General Information

Symbol

Dmel\Rack1^1.8

Species

D. melanogaster

Name

FlyBase ID

FBal0198122

Feature type

allele

Associated gene

Dmel\Rack1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Kadrmas et al., 2007)

Mutagen

ethyl methanesulfonate

(Kadrmas et al., 2007)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Kadrmas et al., 2007)

Amino acid replacement: Q6term.

(Kadrmas et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:7,827,154..7,827,154 [-]

Nucleotide change:

C7827154T

Reported nucleotide change:

C?T

Amino acid change:

Q6term | Rack1-PA; Q6term | Rack1-PB; Q6term | Rack1-PC; Q6term | Rack1-PD

Reported amino acid change:

Q6term

(Kadrmas et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates muscular dystrophy

modeled by Dg^O86

(Kucherenko et al., 2011)

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{63Q.GMR.Tag:HA}

(Xie et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

(Kucherenko et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2L)ED12527)

(Kahsai and Cook, 2018)

lethal | embryonic stage | germline clone

(Kadrmas et al., 2007)

lethal | larval stage | recessive

(Kadrmas et al., 2007)

sterile (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

sterile (with Rack1^EE)

(Kadrmas et al., 2007)

sterile (with Rack1^EY00128)

(Kadrmas et al., 2007)

Phenotype Manifest In

autophagosome

(Érdi et al., 2012)

cuticle (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

cuticle (with Rack1^EE)

(Kadrmas et al., 2007)

cuticle (with Rack1^EY00128)

(Kadrmas et al., 2007)

egg chamber (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

embryonic/larval fat body | larval stage

(Érdi et al., 2012)

female germline stem cell (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

glycogen granule

(Érdi et al., 2012)

ovary (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

ovary (with Rack1^EE)

(Kadrmas et al., 2007)

ovary (with Rack1^EY00128)

(Kadrmas et al., 2007)

ovary | germline clone

(Kadrmas et al., 2007)

ring canal (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

wing (with Df(2L)RACK1-mts)

(Kadrmas et al., 2007)

wing (with Rack1^EE)

(Kadrmas et al., 2007)

wing (with Rack1^EY00128)

(Kadrmas et al., 2007)

Detailed Description

Statement

Reference

Fewer autolysosomes are formed in the fat body cells of Rack1^1.8 mutant larvae during starvation compared to controls, and the ones that are seen are small. The cytoplasmic area covered by autophagosomes and autolysosomes is also decreased. Glycogen stores are also strongly decreased in Rack1^1.8 mutants, with an 11.8 fold reduction in glycogen particle area compared to controls.

(Érdi et al., 2012)

75% of Rack1^1.8 mutants show larval lethality and of those that survive to pupariate, 85% die before adult eclosion.

When females carrying Rack1^1.8 germ-line clones are mated to wild-type males, the females display a dramatically reduced rate of egg lay and aberrantly small ovaries. The produced eggs are reduced in size by an average of 30% compared to wild-type eggs. However, these eggs are competent to be fertilized, although fertilized embryos arrest very early in embryogenesis.

Some Rack1^1.8/Df(2L)RACK1-mts mutant germaria contain numbers of germline stem cells (GSCs) that are within the normal range, while others are completely devoid of GSCs. Ovarioles that contain no GSCs contain more mature egg chambers. Some Rack1^1.8/Df(2L)RACK1-mts egg chambers contain fewer germ cells per chamber and a large portion of chambers contain either four or eight nuclei. Additionally, the number of ring canals does not always correspond to the number of nuclei present.

Rack1^1.8/Df(2L)RACK1-mts, Rack1^1.8/Rack1^EY00128 and Rack1^1.8/Rack1^EE escapers display a range of phenotypes including sluggish movement, difficulty in exiting the pupal case, cuticle that does not harden, and wings that are blistered, abnormally small, or that fail to unfold. Escaper females are incapable of laying eggs and the size of the ovary is reduced compared to wild type but overall body size is unaffected.

(Kadrmas et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Rack1[+]/Rack1^1.8 is a non-enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Rack1[+]/Rack1^1.8 is a non-enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Other

Statement

Reference

Dg[+]/Dg^O86, Rack1^1.8 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Rack1 Dys double heterozygous flies (Rack1^1.8/Df(3R)Exel6184) do not exhibit indirect flight muscle degeneration.

Rack1^1.8 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

One copy of Rack1^1.8 does not enhance the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of Rack1^1.8 does not enhance the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

Rack1^1.8 Dg³²³ double heterozygous flies do not exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Rack1^1.8 is partially rescued by Rack1^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^hs.PB

(Érdi et al., 2012)

Comments

Expression of Rack1^{Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^hs.PB partially rescues the reduction in autolysosome number and size seen in the fat body cells of Rack1^1.8 mutant larvae. The reduction in cytoplasmic area covered by autophagic structures is also partially rescued, as is the depletion of glycogen stores.

(Érdi et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24152

w^*; Rack1^1.8 P{neoFRT}40A/CyO, S²

Notes on Origin

Discoverer

R. Holmgren

(Kadrmas et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

RACK1^1.8

(Kadrmas et al., 2007)

Rack1^1.8

(Chen et al., 2019, Kahsai and Cook, 2018, Érdi et al., 2012, Kucherenko et al., 2011)

rack1^1.8

(Sun et al., 2023, Xie et al., 2021, Li et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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