Lateral horn neurons that normally express Scer\GAL4NP6099 show attenuated odor responses in Ir64aMB05283 mutant flies.
Ir64aMB05283 mutants exhibit a reduced DC4 and DP1M glomeruli response to odorants.
Mutant flies show mild but significant defects in the auditory response to sound compared to wild type.
Homozygous Ir64aMB05283 mutant flies display impaired response to acids in DC4 dorsal glomeruli of the adult antennal lobe and impaired avoidance of acids in behavioural experiments. These mutants also display impaired activation of DP1m neurons to acidic and non-acidic odorants.
In contrast to wild-type flies, homozygous Ir64aMB05283 mutants are equally attracted to vinegar and to neutralised vinegar.
Gr63a1, Ir64aMB05283 has abnormal smell perception | recessive phenotype, suppressible by Scer\GAL4Ir64a.PA/Gr63aUAS.cJa/Gr21aUAS.cJa
Gr63a1, Ir64aMB05283 has abnormal smell perception | recessive phenotype
Expression of both Gr63aScer\UAS.cJa and Gr21aScer\UAS.cJa under the control of Scer\GAL4Ir64a.PA in Ir64aMB05283, Gr63a1 double mutant flies allows artificial stimulation of DC4 dorsal glomeruli of the adult antennal lobe by 5% CO[[2]] and robust avoidance of CO[[2]] in behavioural experiments and robust avoidance of CO[[2]] in behavioural experiments. However, DP1m neurons are not activated by CO[[2]] in these mutants.
Behavioural experiments demonstrate that Gr63a1; Ir64aMB05283 double mutant flies fail to distinguish ambient air from 5% CO[[2]].
Ir64aMB05283 is rescued by Ir64aTag:HA
Ir64aMB05283 is rescued by Scer\GAL4Ir64a.PA/Ir64aUAS.cAa
Ir64aT:Ivir\HA1 rescues the impaired acid response in DC4 glomeruli.
Expression of Ir64aScer\UAS.cAa driven by Scer\GAL4Ir64a.PA rescues the impaired acid response in DC4 glomeruli and the impaired avoidance of acids in behavioural tests.
