FlyBase Allele Report: Dmel\14-3-3ζ[GD16467]

General Information

Symbol

Dmel\14-3-3ζ^GD16467

Species

D. melanogaster

Name

FlyBase ID

FBal0198582

Feature type

allele

Associated gene

Dmel\14-3-3ζ

Associated Insertion(s)

Carried in Construct

P{GD16467}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD16467}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD16467

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Shirasaki et al., 2012)

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

RNAi knockdown of 14-3-3ζ, through expression of 14-3-3ζ^GD16467 in the posterior compartment of the wing disc, under the control of either Scer\GAL4^en.PU or Scer\GAL4^hh.PU, does not affect levels of cell death.

(Wong et al., 2014)

Adults expressing 14-3-3ζ^GD16467 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16467

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

14-3-3ζ^GD16467, Scer\GAL4^elav.PU is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^elav.PU

(Shirasaki et al., 2012)

Other

Statement

Reference

14-3-3ζ^GD16467, Scer\GAL4^hh.PU, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

14-3-3ζ^GD16467, Scer\GAL4^en.PU, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

14-3-3ζ^GD16467, Scer\GAL4^He.PZ is a suppressor of hemocyte phenotype of Scer\GAL4^He.PZ, mtm^RNAi.cVa.UAS

(Pueyo et al., 2016)

14-3-3ζ^GD16467, Scer\GAL4^He.PZ is a suppressor of endolysosome phenotype of Scer\GAL4^He.PZ, mtm^RNAi.cVa.UAS

(Pueyo et al., 2016)

14-3-3ζ^GD16467, Scer\GAL4^He.PZ is a suppressor of hemocyte phenotype of Pi3K68D^UAS.mCherry, Scer\GAL4^He.PZ

(Pueyo et al., 2016)

14-3-3ζ^GD16467, Scer\GAL4^He.PZ is a suppressor of endolysosome phenotype of Pi3K68D^UAS.mCherry, Scer\GAL4^He.PZ

(Pueyo et al., 2016)

Other

Statement

Reference

14-3-3ζ^GD16467, Scer\GAL4^hh.PU, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

14-3-3ζ^GD16467, Scer\GAL4^en.PU, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of 14-3-3ζ^GD16467 under the control of Scer\GAL4^He.PZ rescues the endolysosome phenotype of hemo-A4/hemo-A4 mutant hemocytes.

Co-expression of hemo^Scer\UAS.FL or 14-3-3ζ^GD16467 rescues the increased endolysosomal compartment phenotype of hemocytes expressing Pi3K68D^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^He.PZ.

Co-expression of 14-3-3ζ^GD16467 rescues the increased endolysosomal compartment phenotype of hemocytes expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^He.PZ.

(Pueyo et al., 2016)

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when 14-3-3ζ^GD16467 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant significant increase in cell death in these double mutants. Consistent with high levels of cell death and consequent loss of tissue, adult wings show notching when JNK signalling is reduced in a chromosome instability background (through expression of mad2^GD16427).

(Wong et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v48724