UASt regulatory sequences drive expression of an inverted repeat.
The expression of AstCGD3649 under the control of Scer\GAL4VP16.CNMa leads to absent circadian vitellogenesis rhythm (i.e. absence of peak stage 8 oocyte production at CT14).
Expression of AstCGD3649 under the control of Scer\GAL4nSyb.PU (in combination with UAS-Dicer2 to increase RNAi efficiency) does not result in a significant change in the latency to sperm ejection in females after the end of copulation.
Adults expressing AstCGD3649 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.