FlyBase Allele Report: Dmel\Atx2[GD11562]

General Information

Symbol

Dmel\Atx2^GD11562

Species

D. melanogaster

Name

FlyBase ID

FBal0198800

Feature type

allele

Associated gene

Dmel\Atx2

Associated Insertion(s)

Carried in Construct

P{GD11562}

Also Known As

UAS-Atx2RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11562}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11562

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of chromosome 16p11.2 deletion syndrome, 593-kb

CEA

(Yusuff et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates chromosome 16p11.2 deletion syndrome, 593-kb

modeled by Pp4-19C^GD9561

(Iyer et al., 2018)

modeled by Rph^GD7330

(Iyer et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian behavior, with Scer\GAL4^P2.4.Pdf

(Zhang et al., 2013)

abnormal learning, with Scer\GAL4^GH146

(McCann et al., 2011)

abnormal learning, with Scer\GAL4^GH146, Scer\GAL80^ts.αTub84B

(McCann et al., 2011)

abnormal learning, with Scer\GAL4^VPN

(McCann et al., 2011)

abnormal learning | heat sensitive, with Scer\GAL4^NP1227, Scer\GAL80^ts.αTub84B

(Sudhakaran et al., 2014)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

Detailed Description

Statement

Reference

Expression of Atx2^GD11562 under the control of Scer\GAL4^NP1227 (limited to the adult stages using Scer\GAL80^ts.αTub84B) has no effect on the short term habituation (STH) seen following exposure to either ethyl butyrate (EB) or CO[[2]], but results in a complete block of long term habituation (LTH) to this odorant.

(Sudhakaran et al., 2014)

Expression of Atx2^GD11562 under the control of Scer\GAL4^P2.4.Pdf (co-expressed with Dcr-2^Scer\UAS.cDa) increases the period of circadian locomotor darkness.

(Zhang et al., 2013)

Expression of Atx2^GD11562 under the control of Scer\GAL4^GH146 completely blocks the long-term habituation (LTH) seen when flies are exposed to ethyl butyrate (EB) for 4 days. LTH to CO[[2]] is unaffected. Short term habituation (STH) in response to 1 hour of EB exposure is also unaffected, as is the EB-avoidance response. In contrast to controls, no increase is seen in the volume of either the DM5 glomerulus or the DM2 glomerulus in response to 4h EB exposure. The volume of the V glomerulus increases as in controls. There is a significant reduction in EB-evoked calcium flux compared to controls. A block in EB-specific LTH is also seen when expression of Atx2^GD11562 is restricted to the adult stages using Scer\GAL80^ts.αTub84B.

Expression of Atx2^GD11562 in the CO[[2]]-responsive VPN under the control of Scer\GAL4^VPN selectively blocks LTH to CO[[2]] without altering either STH to CO[[2]] or the naive olfactory response to CO[[2]]. No growth is seen in the volume of the V glomerulus, but normal EB-induced growth of the DM5 glomerulus is seen.

(McCann et al., 2011)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increase in the average size of neuroblasts in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing Atx2^GD11562 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11562

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Atx2^GD11562, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Diap1^RNAi.UAS, Scer\GAL4^GMR.PU

(McCann et al., 2011)

Atx2^GD11562, Scer\GAL4^GMR.PU is a non-suppressor of increased cell death phenotype of Diap1^RNAi.UAS, Scer\GAL4^GMR.PU

(McCann et al., 2011)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Atx2^GD11562, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Diap1^RNAi.UAS, Scer\GAL4^GMR.PU

(McCann et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Atx2^GD11562 does not suppress the cell death phenotype seen in the eyes of flies expressing th^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

(McCann et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v34955