FlyBase Allele Report: Dmel\ICA69[GD7020]

General Information

Symbol

Dmel\ICA69^GD7020

Species

D. melanogaster

Name

FlyBase ID

FBal0199071

Feature type

allele

Associated gene

Dmel\ICA69

Associated Insertion(s)

Carried in Construct

P{GD7020}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7020}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7020

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Mef2.PU

(Mallik et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Mef2.PU

(Mallik et al., 2017)

NMJ bouton | third instar larval stage, with Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

NMJ bouton | third instar larval stage, with Scer\GAL4^Mef2.PU

(Mallik et al., 2017)

Detailed Description

Statement

Reference

Expression of ICA69^GD7020 under the control of either Scer\GAL4^Act5C.PU or Scer\GAL4^Mef2.PU (but not Scer\GAL4^elav-C155) results in significant decrease in the average bouton area, bouton number and in total number of branches in neuromuscular junctions in third instar larvae compared to controls.

ICA69^GD7020 expression driven by either Scer\GAL4^Mef2.PU or Scer\GAL4^elav-C155 does not affect neuromuscular junction synapse retraction in third instar larvae, neither are any synaptic electrophysiology defects observed upon Scer\GAL4^Act5C.PU-driven expression.

(Mallik et al., 2017)

Adults expressing ICA69^GD7020 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7020

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

ICA69^GD7020, Scer\GAL4^Act5C.PU has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by PICK1^KK109273, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

ICA69^GD7020, Scer\GAL4^Act5C.PU has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by Rab2^HMS01271, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

NOT Enhancer of

Statement

Reference

ICA69^GD7020, Scer\GAL4^Act5C.PU is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of PICK1^KK109273, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

ICA69^GD7020, Scer\GAL4^Act5C.PU is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Rab2^HMS01271, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

ICA69^GD7020, Scer\GAL4^Act5C.PU has NMJ bouton | third instar larval stage phenotype, non-enhanceable by PICK1^KK109273, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

ICA69^GD7020, Scer\GAL4^Act5C.PU has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by PICK1^KK109273, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

ICA69^GD7020, Scer\GAL4^Act5C.PU has NMJ bouton | third instar larval stage phenotype, non-enhanceable by Rab2^HMS01271, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

ICA69^GD7020, Scer\GAL4^Act5C.PU has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Rab2^HMS01271, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

NOT Enhancer of

Statement

Reference

ICA69^GD7020, Scer\GAL4^Act5C.PU is a non-enhancer of NMJ bouton | third instar larval stage phenotype of Rab2^HMS01271, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

ICA69^GD7020, Scer\GAL4^Act5C.PU is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rab2^HMS01271, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

ICA69^GD7020, Scer\GAL4^Act5C.PU is a non-enhancer of NMJ bouton | third instar larval stage phenotype of PICK1^KK109273, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

ICA69^GD7020, Scer\GAL4^Act5C.PU is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of PICK1^KK109273, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The neuromuscular junction (NMJ) structural defects (smaller synapse, reduced number of boutons) characteristic for third instar larvae expressing PICK1^KK109273 or Rab2^HMS01271 or ICA69^GD7020 individually under the control of Scer\GAL4^Act5C.PU are not further worsened when either PICK1^KK109273 or Rab2^HMS01271 RNAi is co-expressed with ICA69^GD7020.

However, the Rab2^HMS01271 RNAi-induced NMJ defects can be rescued by co-expression of ICA69^Scer\UAS.FL.

(Mallik et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

The neuromuscular junction structural defects (reduced bouton area and number, decreased total number of branches) observed in ICA69^GS13474/Df(3L)BSC553 third instar larvae is rescued by expression of ICA69^Scer\UAS.FL under the control of either Scer\GAL4^Act5C.PU or Scer\GAL4^Mef2.PU (but not Scer\GAL4^elav-C155).

(Mallik et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v27281

w¹¹¹⁸; P{GD7020}v27281

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts