FB2024_03 , released June 25, 2024
Allele: Dmel\FarOGD3202
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General Information
Symbol
Dmel\FarOGD3202
Species
D. melanogaster
Name
FlyBase ID
FBal0202007
Feature type
allele
Associated gene
Dmel\FarO
Associated Insertion(s)
Carried in Construct
P{GD3202}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD3202}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD3202
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Somatic clones in the larval fat body expressing FarOGD3202 under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C do not display a detectable change in lipid droplets or cell size during starvation, as compared to controls.

      Expression of FarOGD3202 under the control of Scer\GAL4Desat1.PB, or under the control of Scer\GAL4Cyp4g1-NP6073 and Scer\GAL80elav.PU to restrict expression to oenocytes, results in a decrease in the accumulation of lipid droplets in larval oenocytes during starvation, but not in fed conditions, as compared to controls.

      Expression of FarOGD3202 under the control of Scer\GAL4Desat1.PB leads to a significant increase in larval oenocyte size in both fed conditions at 48 hours after hatching, and starved conditions 66 hours after hatching, but there is no difference in oenocyte size during fed conditions 66 hours after hatching, as compared to controls. Oenocyte enlargement during starvation is also associated with enlarged nuclei.

      Somatic clones in larval oenocytes expressing FarOGD3202 under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C display a decrease in lipid droplets, as compared to control cells.

      (Cinnamon et al., 2016)

      Adults expressing CG18031GD3202 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of FarOGD3202 suppresses the increased lipid droplet content of oenocytes seen in fed larvae, and suppresses the decreased oenocyte size of both fed and starved larvae expressing Pi3K92EA2860C.Scer\UAS under the control of Scer\GAL4Desat1.PB.

      (Cinnamon et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG18031GD3202
      FarOGD3202
      Name Synonyms
      Secondary FlyBase IDs
        References (7)