FB2024_03 , released June 25, 2024
Allele: Dmel\SlimpGD10175
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General Information
Symbol
Dmel\SlimpGD10175
Species
D. melanogaster
Name
FlyBase ID
FBal0202745
Feature type
allele
Associated gene
Dmel\Slimp
Associated Insertion(s)
Carried in Construct
P{GD10175}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD10175}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD10175
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4Act5C.PU-mediated expression of SlimpGD10175 significantly reduces viability: only 12% of flies emerge. Lethality is increased at higher temperatures. Lethality is significantly suppressed by addition of an anti-oxidant mix in the diet.

      Scer\GAL4ptc-559.1-mediated expression of SlimpGD10175 results in a narrowing of the region flanked by longitudinal veins L3 and L4 in the adult wing. This is caused by a reduction in the number of cells and not a reduction in cell size. The anterior cross vein is reduced/lost in some cases.

      The relative amount of mitochondrial DNA in Scer\GAL4Act5C.PU, SlimpGD10175 larvae is increased compared to controls.

      Scer\GAL4Act5C.PU-mediated expression of SlimpGD10175 does not reduce mitochondrial respiratory values (normalized by mitochondrial density).

      (Guitart et al., 2010)

      Adults expressing SlimpGD10175 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or early pupal lethality.

      (Schnorrer et al., 2010)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4nub-AC-62-mediated co-expression of SlimpGD10175 and Dcr-2Scer\UAS.cDa results in failure of the wing to unfold, though the overall structure of the wing is not compromised.

      (Guitart et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG31133GD10175
      SlimpGD10175
      Name Synonyms
      Secondary FlyBase IDs
        References (6)