FB2024_03 , released June 25, 2024
Allele: Dmel\chasGD7593
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General Information
Symbol
Dmel\chasGD7593
Species
D. melanogaster
Name
FlyBase ID
FBal0203374
Feature type
allele
Associated gene
Dmel\chas
Associated Insertion(s)
Carried in Construct
P{GD7593}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Olguín et al., 2011, Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Olguín et al., 2011, Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Olguín et al., 2011, Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD7593}
Cytology
Description

UASt regulatory sequences drive expression of a dsRNA that targets both A and B isoforms of chas.

(Olguín et al., 2011)

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD7593
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Tendon cell projections connecting dorsolongitudinal muscles do not elongate at 28-32 hours after puparium formation in animals expressing chasGD7593 under the control of Scer\GAL4pnr-MD237, in contrast to controls.

      (Olguín et al., 2011)

      Expression of chasGD7593 under the control of Scer\GAL4pnr-MD237 in the notum results in notum bristles and trichomes pointing to the midline and multiple trichomes per cell. Epidermal indentations are seen in most anterior notum regions. The anterior indentations are detected by 27-28 hours after puparium formation (APF) and are very obvious by 32 hours APF, coinciding with the attachment domains of the ventral dorsolongitudinal muscles.

      Bristle and socket cells of the notum have rotated towards the midline more than in controls in animals expressing chasGD7593 under the control of Scer\GAL4pnr-MD237 at 32 hours after puparium formation.

      Expression of chasGD7593 under the control of Scer\GAL4pnr-MD237 from 14 hours after puparium formation (APF) (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit repress expression before this stage) results in weak bristle orientation defects in the notum.

      (Olguín et al., 2011)

      Adults expressing chasGD7593 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

      Expression under the control of Scer\GAL4pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 20-30% or 60-70% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

      (Mummery-Widmer et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of jbugGD13033 and chasGD7593 under the control of Scer\GAL4pnr-MD237 results in enhanced indentations of the anterior notum epidermis and enhanced planar polarity defects compared to either single mutant.

      Co-expression of jbugL.Scer\UAS suppresses the planar polarity defects and the anterior epidermal indentations in the notum which are caused by expression of chasGD7593 under the control of Scer\GAL4pnr-MD237.

      Co-expression of chasGD7593 and zipDN.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4pnr-MD237 from 14 hours after puparium formation (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit repress expression before this stage) results in strong bristle orientation defects and epidermal indentations in the notum.

      (Olguín et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG32556GD7593
      chasGD7593
      Name Synonyms
      Secondary FlyBase IDs
        References (8)