Expression of Nlg4^GD3083 under the control of any of the following drivers: Scer\GAL4^ort.C1-4, Scer\GAL4^ChAT.7.4 or Scer\GAL4^oc.1.6 (but not Scer\GAL4^GMR31C06) results in the aberrant projection of axons of the L4 lamina neurons into adjacent columns in the adult medulla. Expression in individual lamina neurons - using Scer\GAL4^c202a, Scer\GAL4^21D, Scer\GAL4^{DBD.R75H07.T:Zzzz\Zip-} combined with Hsap\RELA^{AD.R64B03.T:Zzzz\Zip+} and Scer\GAL4^{DBD.R31H09.T:Zzzz\Zip-} combined with Hsap\RELA^{AD.R21A05.T:Zzzz\Zip+} drivers does not disturb axonal targeting in these neurons.

Expression of Nlg4^GD3083 under the control of Scer\GAL4^RapGAP1-OK6 (but not Scer\GAL4^C57) leads to decreased bouton number and increased bouton size on neuromuscular junctions in third instar larval stage.

Expression of Nlg4^GD3083, under the control of Scer\GAL4^per.PK or Scer\GAL4^P2.4.Pdf leads to abnormal sleep, with reduced seep time and increased sleep latency. Expression under the control of Scer\GAL4^104Y, Scer\GAL4^repo, Scer\GAL4^VGlut-OK371, Scer\GAL4^c819 and Scer\GAL4^pain-GAL4 leads to abnormalities in sleep, although not to a statistically significant extent.

Flies expressing Nlg4^GD3083 under the control of Scer\GAL80^ts.αTub84B and Scer\GAL4^P2.4.Pdf exhibit a progressive delay of sleep onset latency after switching to 30[o]C. Two days after shifting back to 25[o]C, Nlg4^GD3083 Scer\GAL80^ts.αTub84B/Scer\GAL4^P2.4.Pdf flies still show longer sleep onset latency and less total night sleep time, whereas control flies exhibit normal sleep behaviour.

Adults expressing Nlg4^GD3083 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality before the pupal stage, depending on the insertion line used.

Nlg4^GD3083, Scer\GAL4^P2.4.Pdf has decreased sleep | adult stage phenotype, non-enhanceable by Gat^33-1

Nlg4^GD3083, Scer\GAL4^P2.4.Pdf has abnormal sleep | adult stage phenotype, non-enhanceable by Gat^33-1

Nlg4^GD3083, Scer\GAL4^P2.4.Pdf has decreased sleep | adult stage phenotype, non-suppressible by Gat^33-1

Nlg4^GD3083, Scer\GAL4^P2.4.Pdf has abnormal sleep | adult stage phenotype, non-suppressible by Gat^33-1