FlyBase Allele Report: Dmel\Prp40[GD11004]

General Information

Symbol

Dmel\Prp40^GD11004

Species

D. melanogaster

Name

FlyBase ID

FBal0203976

Feature type

allele

Associated gene

Dmel\Prp40

Associated Insertion(s)

Carried in Construct

P{GD11004}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11004}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11004

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell size, with Scer\GAL4^ptc.PU

(Prieto-Sánchez et al., 2020)

decreased size | adult stage, with Scer\GAL4^dpp.PU

(Prieto-Sánchez et al., 2020)

decreased size | adult stage, with Scer\GAL4^en.PU

(Prieto-Sánchez et al., 2020)

decreased size | adult stage, with Scer\GAL4^ey.PU

(Prieto-Sánchez et al., 2020)

decreased size | adult stage, with Scer\GAL4^MD-638

(Prieto-Sánchez et al., 2020)

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

increased cell death | third instar larval stage, with Scer\GAL4^en.PU

(Prieto-Sánchez et al., 2020)

lethal, with Prp40^{ΔWW.UAS.Tag:V5}, Scer\GAL4^en.PU

(Prieto-Sánchez et al., 2020)

lethal - all die during embryonic stage, with Scer\GAL4^Act5C.PU

(Prieto-Sánchez et al., 2020)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^ey.PU

(Prieto-Sánchez et al., 2020)

visible | adult stage, with Scer\GAL4^MD-638

(Prieto-Sánchez et al., 2020)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PU

(Prieto-Sánchez et al., 2020)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

nucleus, with Scer\GAL4^ptc.PU

(Prieto-Sánchez et al., 2020)

posterior crossvein, with Scer\GAL4^dpp.PU

(Prieto-Sánchez et al., 2020)

salivary gland, with Scer\GAL4^ptc.PU

(Prieto-Sánchez et al., 2020)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^dpp.PU

(Prieto-Sánchez et al., 2020)

wing, with Scer\GAL4^en.PU

(Prieto-Sánchez et al., 2020)

wing, with Scer\GAL4^MD-638

(Prieto-Sánchez et al., 2020)

wing disc | third instar larval stage, with Scer\GAL4^en.PU

(Prieto-Sánchez et al., 2020)

Detailed Description

Statement

Reference

Expression of Prp40^GD11004 under the control of Scer\GAL4^MD-638 leads to an extreme reduction in wing size, losing most of their pattern elements.

Expression of Prp40^GD11004 under the control of Scer\GAL4^en.PU leads to increased apoptosis in the third instar wing imaginal disc in specific areas.

Expression of Prp40^GD11004 under the control of Scer\GAL4^dpp.PU or Scer\GAL4^en.PU leads to a reduction in wing size.

Expression of Prp40^GD11004 under the control of Scer\GAL4^ey.PU leads to a size reduction and disorganization in the eye.

Expression of Prp40^GD11004 under the control of Scer\GAL4^ptc.PU leads to a size reduction in salivary gland cells with a reduction in DAPI staining of nuclei.

(Prieto-Sánchez et al., 2020)

Adults expressing CG3542^GD11004 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of CG3542^GD11004 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD11004} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 70-80% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 80-90% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development