FlyBase Allele Report: Dmel\PPP1R15[GD5953]

General Information

Symbol

Dmel\PPP1R15^GD5953

Species

D. melanogaster

Name

FlyBase ID

FBal0204091

Feature type

allele

Associated gene

Dmel\PPP1R15

Associated Insertion(s)

Carried in Construct

P{GD5953}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5953}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5953

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing disc | third instar larval stage, with Scer\GAL4^en-e16E

(Sun et al., 2020)

Detailed Description

Statement

Reference

Expression of PPP1R15^GD5953 under the control of Scer\GAL4^en-e16E leads to increased cell death in third instar larval wing discs.

(Sun et al., 2020)

Expression of Gadd34^GD5953 under the control of either Scer\GAL4^tub.PU or Scer\GAL4^e22c results in significant embryonic lethality associated with the failure of dorsal closure in some cases.

Animals expressing Gadd34^GD5953 under the control of Scer\GAL4^da.G32 show delayed larval development. After 6 days, most of the animals reach the third larval instar stage but are smaller than controls, appear sluggish and often display moulting defects (including retention of previous instar mouth hooks and cuticle shedding).

(Malzer et al., 2013)

Adults expressing Gadd34^GD5953 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in a weak flyer phenotype.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 40-50% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in mild thorax closure defects.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5953

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

PPP1R15^GD5953, Scer\GAL4^da.G32 has abnormal developmental rate | larval stage phenotype, suppressible | partially by PEK^GD5584, Scer\GAL4^da.G32

(Malzer et al., 2013)

Other

Statement

Reference

Gcn2^KK103566, PPP1R15^GD5953, Scer\GAL4^da.G32 has majority die during embryonic stage phenotype

(Malzer et al., 2013)

PEK^GD5584, PPP1R15^GD5953, Scer\GAL4^da.G32 has partially lethal phenotype

(Malzer et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Embryos co-expressing both Gadd34^GD5953 and Gcn2^KK103566 under the control of Scer\GAL4^da.G32 show a strong reduction in the rate of hatching compared to both wild type and embryos expressing either Gadd34^GD5953 or Gcn2^KK103566 singly.

Co-expression of PEK^GD5584 modestly rescues the developmental delay phenotype of animals expressing Gadd34^GD5953 under the control of Scer\GAL4^da.G32. However, these animals often die during eclosion from the pupal case, and surviving adults show severe mobility defects and have strongly reduced longevity.

(Malzer et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v15238

w¹¹¹⁸; P{GD5953}v15238

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5953

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG3825^GD5953

Gadd34^GD5953

PPP1R15^GD5953

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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