FB2024_03 , released June 25, 2024
Allele: Dmel\mfrnGD336
Open Close
General Information
Symbol
Dmel\mfrnGD336
Species
D. melanogaster
Name
FlyBase ID
FBal0204487
Feature type
allele
Associated gene
Dmel\mfrn
Associated Insertion(s)
Carried in Construct
P{GD336}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD336}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD336
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Friedreich ataxia
      modeled by fhRNAi.UAS.cLa
      (Navarro et al., 2015)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of mfrnGD336 under the control of Scer\GAL4Act5C.PU causes lethality in under low iron conditions, whereas flies eclose at normal rates when reared on normal or iron-enriched food.

      (Navarro et al., 2015)

      Expression via P{GAL4-tim.E}27 or P{cry-GAL4.Z}17 (in the presence of Dcr-2Scer\UAS.cDa) does not significantly affect circadian rhythmicity in locomotor activity assays in constant darkness.

      (Mandilaras and Missirlis, 2012)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of mfrnGD336 suppresses the reduced survival seen when fhdsRNA.Scer\UAS.cLa is expressed under the control of Scer\GAL4Act5C.PU in response to 2.5mM iron treatment. The reduced survival seen in fhdsRNA.Scer\UAS.cLa-expressing flies at 15mM iron treatment is not suppressed by mfrnGD336. The reduction in aconitase activity is completely restored.

      Expression of mfrnGD336 enhances the reduction in lifespan seen when fhdsRNA.Scer\UAS.cLa is expressed under the control of Scer\GAL4Act5C.PU on a normal diet.

      Expression of mfrnGD336 suppresses the motor defects and brain vacuolization observed in flies expressing fhdsRNA.IR.Scer\UAS under the control of Scer\GAL4repo.PU.

      (Navarro et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG4963GD336
      mfrnGD336
      Name Synonyms
      Secondary FlyBase IDs
        References (8)