FlyBase Allele Report: Dmel\BCAS2[GD11494]

General Information

Symbol

Dmel\BCAS2^GD11494

Species

D. melanogaster

Name

FlyBase ID

FBal0204494

Feature type

allele

Associated gene

Dmel\BCAS2

Associated Insertion(s)

Carried in Construct

P{GD11494}

Also Known As

UAS-dBCAS2^dsRNA

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11494}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11494

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

increased cell death, with Scer\GAL4^en.PU

(Chen et al., 2013)

lethal, with Scer\GAL4^Act5C.PU

(Chen et al., 2013)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^ap-md544

(Chen et al., 2013)

visible, with Scer\GAL4^Bx-MS1096

(Chen et al., 2013)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chaeta, with Scer\GAL4^ap-md544

(Chen et al., 2013)

mesothoracic tergum, with Scer\GAL4^ap-md544

(Chen et al., 2013)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

scutellum, with Scer\GAL4^ap-md544

(Chen et al., 2013)

scutellum, with Scer\GAL4^Bx-MS1096

(Chen et al., 2013)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^ap-md544

(Chen et al., 2013)

wing, with Scer\GAL4^Bx-MS1096

(Chen et al., 2013)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Chen et al., 2013)

Detailed Description

Statement

Reference

Flies expressing BCAS2^GD11494 under the control of Scer\GAL4^Bx-MS1096 have growth defects in the notum and wings, such as an irregular scutellum margin and twisted and shrunken wings.

Flies expressing BCAS2^GD11494 under the control of Scer\GAL4^ap-md544 have defects such as discoloration of the notum, an irregular scutellum margin, abnormal number and length of bristles and twisted and shrunken wings.

Expression of BCAS2^GD11494 under the control of Scer\GAL4^en.PU results in increased apoptosis in the posterior region of the wing disc.

(Chen et al., 2013)

Adults expressing BCAS2^GD11494 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in adult/pharate lethality or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11494

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

BCAS2^GD11494, Scer\GAL4^en.PU has increased cell death phenotype, suppressible | partially by Hsap\BCAS2^C.UAS, Scer\GAL4^en.PU

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^Act5C.PU has lethal phenotype, suppressible | partially by Hsap\BCAS2^UAS.wt, Scer\GAL4^Act5C.PU

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^Bx-MS1096 has visible phenotype, suppressible | partially by Hsap\BCAS2^UAS.wt, Scer\GAL4^Bx-MS1096

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^ap-md544 has visible phenotype, suppressible | partially by Hsap\BCAS2^UAS.wt, Scer\GAL4^ap-md544

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^Bx-MS1096 has visible phenotype, suppressible | partially by Hsap\BCAS2^C.UAS, Scer\GAL4^Bx-MS1096

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^en.PU has increased cell death phenotype, suppressible by Hsap\BCAS2^UAS.wt, Scer\GAL4^en.PU

(Chen et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

BCAS2^GD11494, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by Hsap\BCAS2^UAS.wt, Scer\GAL4^Bx-MS1096

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^en.PU has wing disc posterior compartment phenotype, suppressible by Hsap\BCAS2^C.UAS, Scer\GAL4^en.PU

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^en.PU has wing disc posterior compartment phenotype, suppressible by Hsap\BCAS2^UAS.wt, Scer\GAL4^en.PU

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^Bx-MS1096 has scutellum phenotype, suppressible by Hsap\BCAS2^UAS.wt, Scer\GAL4^Bx-MS1096

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^ap-md544 has wing phenotype, suppressible | partially by Hsap\BCAS2^UAS.wt, Scer\GAL4^ap-md544

(Chen et al., 2013)

BCAS2^GD11494, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by Hsap\BCAS2^C.UAS, Scer\GAL4^Bx-MS1096

(Chen et al., 2013)

NOT suppressed by

Statement

Reference

BCAS2^GD11494, Scer\GAL4^Bx-MS1096 has scutellum phenotype, non-suppressible by Hsap\BCAS2^C.UAS, Scer\GAL4^Bx-MS1096

(Chen et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\BCAS2^Scer\UAS.wt fully restores the scutellum margin and wing shape in animals expressing BCAS2^GD11494 under the control of Scer\GAL4^Bx-MS1096. Wing veins are only partially recovered in these flies, with the anterior crossvein being missing and the posterior crossvein being truncated.

Co-expression of Hsap\BCAS2^Scer\UAS.wt partially rescues wing shape in animals expressing BCAS2^GD11494 under the control of Scer\GAL4^ap-md544.

Co-expression of Hsap\BCAS2^C.Scer\UAS partially rescues wing deformation in animals expressing BCAS2^GD11494 under the control of Scer\GAL4^Bx-MS1096 : the wings are partially unfolded and wing veins can be seen. However, the notum defects are not rescued.

Co-expression of Hsap\BCAS2^Scer\UAS.wt fully suppresses the increased apoptosis seen in the posterior region of the wing disc in animals expressing BCAS2^GD11494 under the control of Scer\GAL4^en.PU.

Co-expression of Hsap\BCAS2^C.Scer\UAS partially suppresses the increased apoptosis seen in the posterior region of the wing disc in animals expressing BCAS2^GD11494 under the control of Scer\GAL4^en.PU.

(Chen et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v26676

w¹¹¹⁸; P{GD11494}v26676/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11494

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

BCAS2^GD11494

CG4980^GD11494

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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