UASt regulatory sequences drive expression of an inverted repeat.
Expression of mgrGD12016 under the control of either Scer\GAL4insc-Mz1407 (with UAS-Dicer2 to enhance RNAi efficiency) or Scer\GAL4wor.PA (together with Scer\GAL80ase.PN) results in formation of ectopic neuroblasts (either type I or type II, respectively) in third instar larval brains.
Adults expressing mgrGD12016 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or undefined lethality.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 0% or 90-100% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression of mgrGD12016 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4erm.PU in pinsP89 mutant background leads to dedifferentiation of intermediate neural progenitors back into neuroblasts (ectopic type II neuroblasts are observed).