FlyBase Allele Report: Dmel\Ars2[GD12300]

General Information

Symbol

Dmel\Ars2^GD12300

Species

D. melanogaster

Name

FlyBase ID

FBal0205631

Feature type

allele

Associated gene

Dmel\Ars2

Associated Insertion(s)

Carried in Construct

P{GD12300}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12300}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12300

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | heat sensitive, with Scer\GAL4^hs.PB

(Sabin et al., 2009)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

female fertile, with Scer\GAL4^nanos.PG

(Czech et al., 2013)

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal, with Scer\GAL4^Act.PU

(Sabin et al., 2009)

lethal, with Scer\GAL4^da.G32

(Sabin et al., 2009)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

myofibril, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing Ars2^GD12300 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies or late pupal lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in frayed myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with broadened Z-lines in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 80-90% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

Adult flies expressing CG7843^GD12300 under the control of Scer\GAL4^hs.PB (induced using heat shock) succumb to infection with DCV earlier than control flies. The mutant flies show increased production of viral capsid proteins compared to controls.

Adult flies expressing CG7843^GD12300 under the control of Scer\GAL4^hs.PB (induced using heat shock) show a significant increase in mortality after infection with VSV, in contrast to control flies which do not succumb to VSV infection.

(Sabin et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development