FlyBase Allele Report: Dmel\Usp32[GD5871]

General Information

Symbol

Dmel\Usp32^GD5871

Species

D. melanogaster

Name

FlyBase ID

FBal0205836

Feature type

allele

Associated gene

Dmel\Usp32

Associated Insertion(s)

Carried in Construct

P{GD5871}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5871}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5871

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by park^GD5543

(Cornelissen et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^Mef2.PU-mediated expression of CG8334^GD5871 results in a normal appearance of mitochondria in adult indirect flight muscles. Mitochondrial membrane potential in Scer\GAL4^tub.PU CG8334^GD5871 flies is normal, and 2-week-old adult flies show no defects in climbing ability.

(Cornelissen et al., 2014)

No phenotype is observed when CG8334^GD5871 is expressed using Scer\GAL4^sqh.PW or Scer\GAL4^elav.PU.

(Tsou et al., 2012)

Expression of CG8334^GD5871 under the control of Scer\GAL4^bbg-C96 does not result in a wing phenotype.

Expression of CG8334^GD5871 under the control of Scer\GAL4^dpp.PU does not result in a bristle phenotype.

(Zhang et al., 2012)

Adults expressing Usp32^GD5871 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5871

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/Usp32^GD5871 is a suppressor of abnormal locomotor behavior phenotype of Scer\GAL4^Tub.PU, park^GD5543

(Cornelissen et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^Mef2.PU/Usp32^GD5871 is a suppressor of mitochondrion phenotype of Scer\GAL4^Mef2.PU, park^GD5543

(Cornelissen et al., 2014)

Scer\GAL4^Tub.PU/Usp32^GD5871 is a suppressor of mitochondrion phenotype of Scer\GAL4^Tub.PU, park^GD5543

(Cornelissen et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of CG8334^GD5871 largely rescues the mitochondrial defects and defective climbing ability seen park^GD5543 Scer\GAL4^Mef2.PU or park^GD5543 Scer\GAL4^tub.PU flies.

(Cornelissen et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v18981