UASt regulatory sequences drive expression of an inverted repeat.
Flies expressing opmGD3366 during adulthood under the control of Scer\GAL4Act5C.PI and Scer\GAL80ts.αTub84B are viable but female flies lay fewer egg per day compared to controls. Male fertility is also reduced.
Scer\GAL4en.PU-mediated expression of opmGD3366 results in loss of posterior wing margin.
Expression under the control of Scer\GAL41407 results in loss of neuroblasts in the larval brain without any change in size of the remaining neuroblasts.
Expression under the control of Scer\GAL41407 results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.
Adults expressing opmGD3366 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in grossly normal adult indirect flight muscles.
Expression under the control of Scer\GAL4Mef2.PR results in frayed myofibrils in the adult indirect flight muscles.
Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 70-80% of the Scer\GAL4pnr-MD237 expression domain.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 30-40% of the Scer\GAL4pnr-MD237-expressing area of the notum.
opmGD3366 is partially rescued by Scer\GAL4en.PU/opmUAS.Tag:HA
Co-expression of opmScer\UAS.T:Ivir\HA1 partially rescues the wing margin defects seen in opmGD3366, Scer\GAL4en.PU wings.