FlyBase Allele Report: Dmel\CaMKII[GD9506]

General Information

Symbol

Dmel\CaMKII^GD9506

Species

D. melanogaster

Name

FlyBase ID

FBal0206477

Feature type

allele

Associated gene

Dmel\CaMKII

Associated Insertion(s)

Carried in Construct

P{GD9506}

Also Known As

UAS-CaMKII RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9506}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9506

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Shirasaki et al., 2012)

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Oka et al., 2017)

ameliorates neuromuscular disease

modeled by Hsap\TRPV4^R269C.UAS

(Woolums et al., 2020)

modeled by Hsap\TRPV4^UAS.cWa

(Woolums et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

partially lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing CaMKII^GD9506 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD9506} insertion line used.

Adults expressing CaMKII^GD9506 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

Expression of CaMKII^GD9506 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD9506} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9506

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

CaMKII^GD9506, Scer\GAL4^elav.PU is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^elav.PU

(Shirasaki et al., 2012)

Suppressor of

Statement

Reference

CaMKII^GD9506, Scer\GAL4^CCAP.PU is a suppressor | partially of visible | adult stage phenotype of Hsap\TRPV4^R269C.UAS, Scer\GAL4^CCAP.PU

(Woolums et al., 2020)

Phenotype Manifest In

Enhancer of

Statement

Reference

CaMKII^GD9506, Scer\GAL4^GMR.PU is an enhancer of lamina phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PU

(Oka et al., 2017)

Suppressor of

Statement

Reference

CaMKII^GD9506, Scer\GAL4^CCAP.PU is a suppressor | partially of wing phenotype of Hsap\TRPV4^R269C.UAS, Scer\GAL4^CCAP.PU

(Woolums et al., 2020)

CaMKII^GD9506, Scer\GAL4^GMR.PU is a suppressor of lamina phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PU

(Oka et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of CaMKII^GD9506 partially suppresses the lamina vacuolization induced by the expression of Hsap\MAPT^Scer\UAS.cWa under the control of Scer\GAL4^GMR.PU.

(Oka et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v38930

w¹¹¹⁸ P{GD9506}v38930

v47280

w¹¹¹⁸; P{GD9506}v47280