FlyBase Allele Report: Dmel\Cyp18a1[GD1176]

General Information

Symbol

Dmel\Cyp18a1^GD1176

Species

D. melanogaster

Name

FlyBase ID

FBal0206724

Feature type

allele

Associated gene

Dmel\Cyp18a1

Associated Insertion(s)

Carried in Construct

P{GD1176}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1176}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1176

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal - all die before end of pupal stage, with Scer\GAL4^da.G32

(Rewitz et al., 2010)

some die during pupal stage, with Scer\GAL4^da.G32

(Rewitz et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult head | pupal stage, with Scer\GAL4^da.G32

(Rewitz et al., 2010)

leg | pupal stage, with Scer\GAL4^da.G32

(Rewitz et al., 2010)

leg | pupal stage, with Scer\GAL4^da.G32, Scer\GAL80^ts.αTub84B

(Rewitz et al., 2010)

Detailed Description

Statement

Reference

Adults expressing Cyp18a1^GD1176 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Animals expressing Cyp18a1^GD1176 under the control of Scer\GAL4^da.G32 display metamorphosis defects, and 95% show pupal lethality. During the prepupal to pupal transition, animals die in three phenotypic classes. Of those that pupariate, 72% of animals die as prepupae or as early pupae after delayed head eversion. A small proportion of mutants die as pharate adults, and a very small number of adult escapers are observed. All animals that pupate display a malformed leg phenotype, and some also show displayed head eversion defects.

87% of animals expressing Cyp18a1^GD1176 under the control of Scer\GAL4^da.G32 and Scer\GAL80^ts.αTub84B at 25[o]C display a leg malformation phenotype.

(Rewitz et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in a weak flyer phenotype.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1176

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cyp18a1^GD1176, Scer\GAL4^da.G32 has lethal | pupal stage phenotype, suppressible by ftz-f1^hs.PM

(Rewitz et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cyp18a1^GD1176, Scer\GAL4^da.G32 has leg | pupal stage phenotype, suppressible by ftz-f1^hs.PM

(Rewitz et al., 2010)

Cyp18a1^GD1176, Scer\GAL4^da.G32, Scer\GAL80^ts.αTub84B has leg | pupal stage phenotype, suppressible by ftz-f1^hs.PM

(Rewitz et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Induction of expression of ftz-f1^hs.PM at 25[o]C in animals expressing Cyp18a1^GD1176 under the control of Scer\GAL4^da.G32 rescues lethality and leg abnormalities.

Induction of expression of ftz-f1^hs.PM at 25[o]C in animals expressing Cyp18a1^GD1176 under the control of Scer\GAL4^da.G32 and Scer\GAL80^ts.αTub84B rescues the leg malformation phenotype. Only 12% of animals display any leg defects, compared to 87% of control animals.

(Rewitz et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v5601

w¹¹¹⁸; P{GD1176}v5601

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1176

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Cyp18a1^GD1176

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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