UASt regulatory sequences drive expression of an inverted repeat.
The expression of E2f1GD4448 under the control of Scer\GAL4NP1624 induces a decrease in the number of hub cells in the testes; similarly, adulthood-only expression (under the additional control of Gal80{ts]) progressive leads to the complete loss of hub cells.
Expression via "HHLT-gal4" results in loss of progenitor cells in the primary lobes of the larval lymph gland. ("HHLT-gal4" is a combination of Scer\GAL4HCH.Hand and Scer\GAL4Hml.Δ that express in early lymph gland blood cells (hemocytes) and circulating and sessile blood cells, respectively, and incorporates Scer\GAL4Scer\FRT.Rnor\CD2.Act5C to maintain expression in the lymph gland after Scer\GAL4HCH.Hand is down-regulated.)
Adults expressing E2f1GD4448 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4pnr-MD237 expression domain.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 0% or 60-70% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.