FlyBase Allele Report: Dmel\EcR[GD1428]

General Information

Symbol

Dmel\EcR^GD1428

Species

D. melanogaster

Name

FlyBase ID

FBal0206933

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

Carried in Construct

P{GD1428}

Also Known As

UAS-EcR^RNAi, UAS-EcR RNAi, UAS-EcR-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1428}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1428

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | P-stage, with Scer\GAL4^A58

(Heredia et al., 2021)

abnormal body size | P-stage, with Scer\GAL4^MsrA.657

(Heredia et al., 2021)

abnormal courtship behavior, with Scer\GAL4^fru.P1.D

(Chen et al., 2022)

abnormal courtship behavior, with Scer\GAL4^Or67d.PU

(Chen et al., 2022)

abnormal neuroanatomy, with Scer\GAL4^109(2)80

(Kirilly et al., 2009)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Homem et al., 2014)

abnormal neuroanatomy | adult stage, with Scer\GAL4^Or67d.PU

(Chen et al., 2022)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Homem et al., 2014)

lethal, with Scer\GAL4^GMR.PF

(Zhu et al., 2013)

lethal, with Scer\GAL4^ple.PF

(Zhu et al., 2013)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal | nutrition conditional, with Scer\GAL4^r4

(Musselman et al., 2018)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Ovaries from virgin females expressing EcR^GD1428 under the control of Scer\GAL4^C587 do not show changes in the number of germline stem cells in the germarium, as compared to controls.

(Ameku et al., 2018)

The expression of EcR^GD1428 under the control of Scer\GAL4^r4 leads to lethality when individuals are reared under a high sugar diet (i.e. 1M sucrose), but not under a standard sugar diet (0.15M sucrose).

(Musselman et al., 2018)

Expression of EcR^GD1428 under the control of Scer\GAL4^phm.PO leads to accumulation of lipid droplets in the prothoracic gland cells of third instar larvae.

(Danielsen et al., 2016)

Expression of EcR^GD1428 under the control of Scer\GAL4^Bx-MS1096 results in male flies with weak wing size and patterning defects.

Expression of EcR^GD1428 under the control of Scer\GAL4^Bx-MS1096 results in female flies with weak wing size and patterning defects.

(Schertel et al., 2015)

Expression via Scer\GAL4^ey-OK107 prevents proper differentiation of alpha/beta neurons in mushroom bodies, resulting in a smaller volume.

Expression via Scer\GAL4^wor.PA and Scer\GAL80^ase.PN prolongs the lifetime and prevents shrinkage of pupal neuroblasts (NBs). These longer lived NBs continue dividing for a longer time and generate more neuronal progeny than wild type.

(Homem et al., 2014)

Larvae expressing EcR^GD1428 under the control of Scer\GAL4^Hml.PG show normal rates of encapsulation of eggs when parasitised by the avirulent L. boulardi wasp strain G486.

(Howell et al., 2012)

Adults expressing EcR^GD1428 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing EcR^GD1428 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

Downregulation of EcR through expression of EcR^GD1428 in ddaC neurons under the control of Scer\GAL4^109(2)80 causes strong ddaC pruning defects at 18h APF.

(Kirilly et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality (either before or at the pupal stage), depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1428

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The G9a^Scer\UAS.cKa wing vein phenotype is almost completely suppressed by co-expression of EcR^GD1428.

(Kleefstra et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v37058

w¹¹¹⁸; P{GD1428}v37058

v37059

w¹¹¹⁸; P{GD1428}v37059

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1428

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EcR^GD1428

EcR^RNAi-3

(Hadjieconomou et al., 2020)

EcR^RNAi

(Ameku et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (32)

Report Sections

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