FlyBase Allele Report: Dmel\Gga[GD1710]

General Information

Symbol

Dmel\Gga^GD1710

Species

D. melanogaster

Name

FlyBase ID

FBal0207111

Feature type

allele

Associated gene

Dmel\Gga

Associated Insertion(s)

Carried in Construct

P{GD1710}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1710}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1710

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^Act5C.PU

(Eissenberg et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

No adult flies expressing P{GD1710} under the control of Scer\GAL4^Act5C.PU in a Gga^Δ background are recovered. This illustrates that the lethality associated with expression of P{GD1710} under the control of Scer\GAL4^Act5C.PU must be an off-target effect (not due to an effect on Gga), since the Gga sequences targeted by the P{GD1710} construct are completely deleted in the Gga^Δ allele.

(Luan et al., 2012)

When Gga^GD1710 is expressed under the control of Scer\GAL4^Act5C.PU, fewer flies than expected survive to adulthood.

(Eissenberg et al., 2011)

Flies expressing Gga^GD1710 ubiquitously under the control of Scer\GAL4^αTub84B.PL develop normally, with no gross morphological changes. There are no differences in fertility in both males and females, neither in rate, number or overall development of progeny. There are no qualitative morphological differences in the development of sperm.

(Hirst and Carmichael, 2011)

Adults expressing Gga^GD1710 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1710

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Gga^GD1710, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Gga^UAS.GFP, Scer\GAL4^GMR.PF

(Eissenberg et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Gga^GD1710, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Gga^UAS.GFP, Scer\GAL4^GMR.PF

(Eissenberg et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Gga^GD1710 under the control of Scer\GAL4^GMR.PF ameliorates the rough eye phenotype caused by Gga^{Scer\UAS.T:Avic\GFP}-overexpression.

(Eissenberg et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v3270