FlyBase Allele Report: Dmel\Lsd-1[GD6641]

General Information

Symbol

Dmel\Lsd-1^GD6641

Species

D. melanogaster

Name

FlyBase ID

FBal0207520

Feature type

allele

Associated gene

Dmel\Lsd-1

Associated Insertion(s)

Carried in Construct

P{GD6641}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6641}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6641

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^fat

(Men et al., 2016)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

atrophy | adult stage, with Scer\GAL4^Bx-MS1096

(Men et al., 2016)

increased cell death | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Men et al., 2016)

lethal, with Scer\GAL4^en.PU

(Men et al., 2016)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal | heat sensitive, with Scer\GAL4^fat

(Men et al., 2016)

lethal - all die during embryonic stage, with Scer\GAL4^Act5C.PU

(Men et al., 2016)

lethal - all die during embryonic stage, with Scer\GAL4^Tub.PU

(Men et al., 2016)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Men et al., 2016)

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

mitochondrion | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Men et al., 2016)

wing, with Scer\GAL4^Bx-MS1096

(Men et al., 2016)

wing pouch, with Scer\GAL4^Bx-MS1096

(Men et al., 2016)

Detailed Description

Statement

Reference

Expression of Lsd-1^GD6641 under the control of Scer\GAL4^Bx-MS1096 results in a severe atrophied wing phenotype in the adult, and significantly increases apoptosis, autophagy, reactive oxygen species activity, and results in enlarged mitochondria in the wing pouch compartment of the wing disc in third instar larvae as compared with controls.

Expression of Lsd-1^GD6641 under the control of Scer\GAL4^en.PU results in lethality.

Expression of Lsd-1^GD6641 under the control of Scer\GAL4^GMR.PU results in no detectable phenotype.

Expression of Lsd-1^GD6641 under the control of Scer\GAL4^fat results in delayed growth when flies are reared at 25[o]C, but is lethal when flies are reared at 28[o]C.

Expression of Lsd-1^GD6641 under the control of Scer\GAL4^Act5C.PU or Scer\GAL4^tub.PU results in lethality at the embryonic stage.

(Men et al., 2016)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing Lsd-1^GD6641 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Lsd-1^GD6641 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD6641} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 100% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6641

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lsd-1^GD6641, Scer\GAL4^Bx-MS1096 has atrophy | adult stage phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^Bx-MS1096

(Men et al., 2016)

Lsd-1^GD6641, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^Bx-MS1096

(Men et al., 2016)

Lsd-1^GD6641, Scer\GAL4^Bx-MS1096 has atrophy | adult stage phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^Bx-MS1096

(Men et al., 2016)

Lsd-1^GD6641, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^Bx-MS1096

(Men et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lsd-1^GD6641, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^Bx-MS1096

(Men et al., 2016)

Lsd-1^GD6641, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by BacA\p35^UAS.cUa, Scer\GAL4^Bx-MS1096

(Men et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Diap1^Scer\UAS.cUa partially rescues the wing atrophy phenotype of flies expressing Lsd-1^GD6641 under the control of Scer\GAL4^Bx-MS1096.

(Men et al., 2016)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cUa partially rescues the wing atrophy phenotype of flies expressing Lsd-1^GD6641 under the control of Scer\GAL4^Bx-MS1096.

(Men et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v30884

w¹¹¹⁸; P{GD6641}v30884

Notes on Origin

Discoverer