UASt regulatory sequences drive expression of an inverted repeat.
The expression of PfkGD1508 from the onset of adulthood, under the combined control of Scer\GAL4da.PU and Gal80[ts], leads to decreased lifespan.
When fed on a low carbohydrate diet (0% sucrose supplement), no developmental delay is observed for Scer\GAL4Cg.PA PfkGD1508 (plus Dcr-2Scer\UAS.cDa) animals. In contrast, when fed a 10% or 20% sucrose supplemented diet, these animals exhibit a significant delay.
The size of cells in flip-out Scer\GAL4Act5C.PP PfkGD1508 (plus Dcr-2Scer\UAS.cDa) clones remains the same size irrespective of sucrose supplementation.
Adults expressing PfkGD1508 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in flightless flies.
Scer\GAL4da.PU/PfkGD1508 is a suppressor | partially of increased cell size | third instar larval stage phenotype of Pten117
PfkGD1508, Scer\GAL4repo is a suppressor | partially of abnormal wound healing | larval stage phenotype of Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS), Scer\GAL4repo, btl::Egfrλ.UAS
PfkGD1508, Scer\GAL4repo is a suppressor | partially of abnormal wound healing | larval stage phenotype of MycUAS.cZa, Scer\GAL4repo
Scer\GAL4da.PU/PfkGD1508 is a non-suppressor of increased cell size | third instar larval stage phenotype of RhebEP50.084-loxP, Scer\GAL4Tub.PU
Scer\GAL4da.PU/PfkGD1508 is a suppressor | partially of larval fat cell | somatic clone | third instar larval stage phenotype of Pten117
PfkGD1508, Scer\GAL4repo is a suppressor | partially of larval multidendritic class IV neuron phenotype of Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS), Scer\GAL4repo, btl::Egfrλ.UAS
PfkGD1508, Scer\GAL4repo is a suppressor | partially of larval multidendritic class IV neuron phenotype of MycUAS.cZa, Scer\GAL4repo
Scer\GAL4da.PU/PfkGD1508 is a non-suppressor of larval fat cell | somatic clone | third instar larval stage phenotype of RhebEP50.084-loxP, Scer\GAL4Tub.PU
The size of cells in Df(2L)FASNΔ24-23 MARCM clones expressing PfkGD1508 (plus Dcr-2Scer\UAS.cDa) is hardly reduced in larvae fed a low carbohydrate diet and remain unaffected in larvae fed a 20% sucrose supplemented diet.