FlyBase Allele Report: Dmel\PTPMT1[GD4965]

General Information

Symbol

Dmel\PTPMT1^GD4965

Species

D. melanogaster

Name

FlyBase ID

FBal0208095

Feature type

allele

Associated gene

Dmel\PTPMT1

Associated Insertion(s)

Carried in Construct

P{GD4965}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4965}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4965

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates inclusion body myopathy with Paget disease of bone and frontotemporal dementia

modeled by TER94^R188Q.UAS

(Chang et al., 2011)

modeled by TER94^A229E.UAS

(Chang et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult tracheal section, with Scer\GAL4^Act5C.PU

(Papakyrikos et al., 2020)

embryonic/larval tracheal section | second instar larval stage, with Scer\GAL4^btl.PS

(Papakyrikos et al., 2020)

embryonic/larval tracheal section | third instar larval stage, with Scer\GAL4^Act5C.PU

(Papakyrikos et al., 2020)

embryonic/larval tracheal section | third instar larval stage, with Scer\GAL4^btl.PS

(Papakyrikos et al., 2020)

embryonic/larval tracheocyte | larval stage, with Scer\GAL4^btl.PS

(Papakyrikos et al., 2020)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

taenidial fold | third instar larval stage, with Scer\GAL4^Act5C.PU

(Papakyrikos et al., 2020)

taenidial fold | third instar larval stage, with Scer\GAL4^btl.PS

(Papakyrikos et al., 2020)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of PTPMT1^GD4965 under the control of Scer\GAL4^Act5C.PU leads to increased mortality during development. Specifically, expression of RNAi 1 leads to larval and pupal lethality (none survives to adulthood) while expression of RNAi 2 leads to some pupal lethality with some adult survivors.

Expression of PTPMT1^GD4965 under the control of Scer\GAL4^Act5C.PU leads to tracheal blackening in adults and in third instar larvae.

Expression of PTPMT1^GD4965 under the control of Scer\GAL4^btl.PS leads to tracheal blackening in second instar larvae.

First instar larvae expressing PTPMT1^GD4965 under the control of Scer\GAL4^btl.PS fail to fill their trachea with air upon completion of molting.

Third instar larvae expressing PTPMT1^GD4965 under the control of Scer\GAL4^Act5C.PU or Scer\GAL4^btl.PS exhibit tracheal lumina filled with unidentifiable membranous materials and large electron-dense regions between the tracheal cuticle and the tracheal epithelial cells.

Expression of PTPMT1^GD4965 under the control of Scer\GAL4^btl.PS leads to mitochondrial aggregates (enlarged mito-GFP puncta) in tracheal cells of first instar larvae just prior to molting and recently molted second instars. A third instar larva exhibit individual mitochondria with intact crista structure aggregated in a tracheal cell.

(Papakyrikos et al., 2020)

Expression of Plip^GD4965 under the control of Scer\GAL4^ninaE.PT has no phenotypic effect on outer photoreceptor cells.

(Chang et al., 2011)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain associated with increased size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of ganglion mother cells in the larval brain.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing Plip^GD4965 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in a weak flyer phenotype.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 60-70% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 30-40% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 40-50% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4965

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

PTPMT1^GD4965, Scer\GAL4^btl.PS has increased mortality during development phenotype, suppressible | partially by MP1^GD5141, Scer\GAL4^btl.PS

(Papakyrikos et al., 2020)

NOT Enhancer of

Statement

Reference

PTPMT1^GD4965, Scer\GAL4^ninaE.PT is a non-enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^ninaE.PT, TER94^K2A.UAS

(Chang et al., 2011)

Suppressor of

Statement

Reference

PTPMT1^GD4965, Scer\GAL4^ninaE.PT is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^ninaE.PT, TER94^R188Q.UAS

(Chang et al., 2011)

PTPMT1^GD4965, Scer\GAL4^ninaE.PT is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^ninaE.PT, TER94^A229E.UAS

(Chang et al., 2011)

NOT Suppressor of

Statement

Reference

PTPMT1^GD4965, Scer\GAL4^ninaE.PT is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^ninaE.PT, TER94^K2A.UAS

(Chang et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

PTPMT1^GD4965, Scer\GAL4^Act5C.PU has adult tracheal section phenotype, suppressible | partially by MP1^GD5141, Scer\GAL4^Act5C.PU

(Papakyrikos et al., 2020)

NOT suppressed by

Statement

Reference

PTPMT1^GD4965, Scer\GAL4^btl.PS has embryonic/larval tracheal section | larval stage phenotype, non-suppressible by MP1^GD5141, Scer\GAL4^btl.PS

(Papakyrikos et al., 2020)

NOT Enhancer of

Statement

Reference

PTPMT1^GD4965, Scer\GAL4^ninaE.PT is a non-enhancer of outer photoreceptor cell phenotype of Scer\GAL4^ninaE.PT, TER94^K2A.UAS

(Chang et al., 2011)

Suppressor of

Statement

Reference

PTPMT1^GD4965, Scer\GAL4^ninaE.PT is a suppressor of outer photoreceptor cell phenotype of Scer\GAL4^ninaE.PT, TER94^R188Q.UAS

(Chang et al., 2011)

PTPMT1^GD4965, Scer\GAL4^ninaE.PT is a suppressor of outer photoreceptor cell phenotype of Scer\GAL4^ninaE.PT, TER94^A229E.UAS

(Chang et al., 2011)

NOT Suppressor of

Statement

Reference

PTPMT1^GD4965, Scer\GAL4^ninaE.PT is a non-suppressor of outer photoreceptor cell phenotype of Scer\GAL4^ninaE.PT, TER94^K2A.UAS

(Chang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Plip^GD4965 under the control of Scer\GAL4^ninaE.PT significantly suppresses the TER94^{R188Q.Scer\UAS} photoreceptor cell phenotype.

Co-expression of Plip^GD4965 under the control of Scer\GAL4^ninaE.PT significantly suppresses the TER94^{A229E.Scer\UAS} photoreceptor cell phenotype.

Co-expression of Plip^GD4965 under the control of Scer\GAL4^ninaE.PT has no significant effect on TER94^K2A.Scer\UAS-induced photoreceptor cell degradation.

(Chang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Co-expression of Plip^GD4965 under the control of Scer\GAL4^ninaE.PT has no significant effect on Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1}-induced photoreceptor cell degradation.

(Chang et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v47624

w¹¹¹⁸; P{GD4965}v47624

Notes on Origin

Discoverer