UASt regulatory sequences drive expression of an inverted repeat.
Scer\GAL4rn-GAL4-5-mediated expression of RpL7GD11462 induces a robust delay in the larva-to-pupa transition of about 3-5 days. The discs grow and mature significantly slower than control discs and reach normal size after the extended period of growth. Accordingly, the larvae grow at a slower rate and reach normal larvae and pupae sizes after an extended period of growth.
Adults expressing RpL7GD11462 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in early larval lethality.
Expression under the control of Scer\GAL4Mef2.PR results in grossly normal larval body wall muscles.
Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.
Expression under the control of Scer\GAL4pnr-MD237 results in lethality before the pupal stage.
RpL7GD11462, Scer\GAL4rn-GAL4-5 has abnormal developmental rate phenotype, suppressible by Ilp8KK112161, Scer\GAL4rn-GAL4-5
RpL7GD11462, Scer\GAL4rn-GAL4-5 has abnormal developmental rate phenotype, suppressible by pucUAS.cMa, Scer\GAL4rn-GAL4-5
Coexpression of Ilp8KK112161 rescues the delay at pupariation of Scer\GAL4rn-GAL4-5, RpL7GD11462 animals.
Coexpression of pucScer\UAS.cMa rescues the delay at pupariation of Scer\GAL4rn-GAL4-5, RpL7GD11462 animals.