FlyBase Allele Report: Dmel\Sod1[GD7385]

General Information

Symbol

Dmel\Sod1^GD7385

Species

D. melanogaster

Name

FlyBase ID

FBal0208571

Feature type

allele

Associated gene

Dmel\Sod1

Associated Insertion(s)

Carried in Construct

P{GD7385}

Also Known As

UAS-SOD1^RNAi, UAS-Sod-IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7385}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7385

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 1

CEC

(Cacciottolo et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 1

modeled by Sod1^GD7385

(Cacciottolo et al., 2019)

model of amyotrophic lateral sclerosis type 1

is exacerbated by Sod1^GD7385

(Cacciottolo et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^188Y

(Martin et al., 2009)

abnormal locomotor behavior | adult stage, with Scer\GAL4^da.G32

(Martin et al., 2009)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PR

(Martin et al., 2009)

abnormal locomotor behavior | larval stage, with Scer\GAL4^C57

(Peng et al., 2019)

short lived, with Scer\GAL4^188Y

(Martin et al., 2009)

short lived, with Scer\GAL4^da.G32

(Martin et al., 2009)

short lived, with Scer\GAL4^elav-C155

(Martin et al., 2009)

short lived, with Scer\GAL4^how-24B

(Martin et al., 2009)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

ISN innervated muscle cell of A1-7 | larval stage, with Scer\GAL4^C57

(Peng et al., 2019)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

The expression of Sod1^GD7385 under the control of Scer\GAL4^C57 leads to decreased muscle contractility, associated with impaired larval locomotion, despite of no obvious changes in neuromuscular junction neurotransmission (i.e. EJP and mEJP amplitude, quantal content, burst duration and frequency, or intra-burst spike density).

(Peng et al., 2019)

Adults expressing Sod^GD7385 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Sod^GD7385 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression of Sod^GD7385 under the control of Scer\GAL4^da.G32 results in a decrease in mean life span. Age related impairment in negative geotaxis is dramatically accelerated in these flies compared to controls.

Expression of Sod^GD7385 under the control of Scer\GAL4^elav-C155, Scer\GAL4^188Y or Scer\GAL4^how-24B results in a small but statistically discernible decrease in life span compared to controls.

Expression of Sod^GD7385 under the control of Scer\GAL4^Appl.G1a, Scer\GAL4^91Y or Scer\GAL4^Mef2.PR has no significant effect on life span.

Expression of Sod^GD7385 under the control of Scer\GAL4^188Y subtly but significantly impairs negative geotaxis across age relative to controls.

Expression of Sod^GD7385 under the control of Scer\GAL4^elav-C155, Scer\GAL4^Appl.G1a, Scer\GAL4^91Y or Scer\GAL4^how-24B has no effect on age related impairment in negative geotaxis.

Expression of Sod^GD7385 under the control of Scer\GAL4^Mef2.PR results in a subtle but statistically discernible acceleration of age related impairment in negative geotaxis compared to controls.

(Martin et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 0-10% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7385

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^Ugt36A1.PK, Sod1^GD7385, Sod2^GD14064 has abnormal oxidative stress response phenotype

(Lim et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^Ugt36A1.PK, Sod1^GD7385, Sod2^GD14064 has adult heart phenotype

(Lim et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Sod^GD7385 and Sod2^GD14064 in nonmyocytic pericardial cells, under the control of Scer\GAL4^Dot.PK results in an abnormal heart rhythm, with an increased frequency of irregular beats, a broader distribution of HPs and significantly increased the arrhythmia index. There is significant cardiac narrowing, with significant reductions in both the diastolic and systolic diameters of young and old flies compared with control flies.

(Lim et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v31551