FlyBase Allele Report: Dmel\Sos[GD1539]

General Information

Symbol

Dmel\Sos^GD1539

Species

D. melanogaster

Name

FlyBase ID

FBal0208578

Feature type

allele

Associated gene

Dmel\Sos

Associated Insertion(s)

Carried in Construct

P{GD1539}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1539}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1539

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

glial cell, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

(Lu et al., 2014)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

vesicle, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

(Lu et al., 2014)

Detailed Description

Statement

Reference

Adult glia expressing Sos^GD1539 (through Scer\GAL4^repo and Scer\GAL80^ts.αTub84B) exhibit a significant delay in engulfment of axonal debris (up to 20 days after axotomy).

(Lu et al., 2014)

Adults expressing Sos^GD1539 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 70-80% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 30-40% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1539

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Sos^GD1539/Scer\GAL4^GMR.PFa is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^repo, Scer\GAL80^ts.αTub84B, Sos^GD1539 has glial cell phenotype, enhanceable by mbc^GD6965/Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

(Lu et al., 2014)

Scer\GAL4^repo, Scer\GAL80^ts.αTub84B, Sos^GD1539 has vesicle phenotype, enhanceable by mbc^GD6965/Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

(Lu et al., 2014)

Enhancer of

Statement

Reference

Sos^GD1539/Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Scer\GAL4^repo/Sos^GD1539, Scer\GAL80^ts.αTub84B is an enhancer of glial cell phenotype of Scer\GAL4^repo, Scer\GAL80^ts.αTub84B, mbc^GD6965

(Lu et al., 2014)

Scer\GAL4^repo/Sos^GD1539, Scer\GAL80^ts.αTub84B is an enhancer of vesicle phenotype of Scer\GAL4^repo, Scer\GAL80^ts.αTub84B, mbc^GD6965

(Lu et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa is exacerbated further by co-expression of Sos^GD1539 RNAi resulting in a 'no eye' phenotype with necrosis-like tissue present.

(Ruan et al., 2016)

Adult glia expressing Sos^GD1539 and mbc^GD6965 (through Scer\GAL4^repo and Scer\GAL80^ts.αTub84B) exhibit complete blockage of glial recruitment to axonal injury sites. This results in the accumulation of axonal debris and a glial hypertrophy response.

(Lu et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v42848

w¹¹¹⁸; P{GD1539}v42848

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1539

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Sos^GD1539

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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