UASt regulatory sequences drive expression of an inverted repeat.
Co-expression of Sp1dsRNA.Scer\UAS, Sp1GD4097 and Dcr-2Scer\UAS.cDa under the control of Scer\GAL4dac-p7d23 (restricted to second instar stage onwards using Scer\GAL80ts.αTub84B) results in a strong reduction in the size of the medial part of the leg.
Co-expression of Sp1dsRNA.Scer\UAS, Sp1GD4097 and Dcr-2Scer\UAS.cDa under the control of Scer\GAL4Dll-em212 (restricted to second instar stage onwards using Scer\GAL80ts.αTub84B) results in a strong reduction in the size of the distal part of the leg.
Co-expression of Sp1dsRNA.Scer\UAS, Sp1GD4097 and Dcr-2Scer\UAS.cDa under control of Scer\GAL4btd-MD808 (restricted to second instar stage onwards using Scer\GAL80ts.αTub84B) results in loss of the antennal a1 and a2 segments.
Co-expression of Sp1dsRNA.Scer\UAS, Sp1GD4097 and Dcr-2Scer\UAS.cDa under control of Scer\GAL4btd-MD808 from embryogenesis onwards results in a reduction in the size of the entire leg, from coxa to the distal tip.
Adults expressing Sp1GD4097 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.