FlyBase Allele Report: Dmel\Sp1[RNAi.UAS]

General Information

Symbol

Dmel\Sp1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0155796

Feature type

allele

Associated gene

Dmel\Sp1

Associated Insertion(s)

Carried in Construct

P{UAS-Sp1-RNAi}

Also Known As

UAS-Sp1i

Key Links

Transgenic product class

RNAi_reagent

(Estella et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Estella et al., 2003)

Mutagen

in vitro construct

(Estella et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Sp1-RNAi}

Cytology

Description

UAS sequences drive expression of a 813 fragment of the Sp1 gene inserted as an inverted repeat.

(Estella et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sp1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^btd-MD808, Scer\GAL80^ts.αTub84B, Sp1^GD4097

(Estella and Mann, 2010)

abnormal size, with Scer\GAL4^btd-MD808, Sp1^GD4097

(Estella and Mann, 2010)

abnormal size, with Scer\GAL4^dac-p7d23, Scer\GAL80^ts.αTub84B, Sp1^GD4097

(Estella and Mann, 2010)

abnormal size, with Scer\GAL4^Dll-em212, Scer\GAL80^ts.αTub84B, Sp1^GD4097

(Estella and Mann, 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Sp1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ap.PU results in the absence of the joint between tarsal segments 4 and 5, with consequent segment fusion.

(Córdoba et al., 2016)

No defects in dendrite pruning of ddaC dorsal dendritic arborising neurons are seen 18 hours after puparium formation in animals expressing Sp1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ppk.1.9.

(Loncle and Williams, 2012)

Co-expression of Sp1^{dsRNA.Scer\UAS}, Sp1^GD4097 and Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^dac-p7d23 (restricted to second instar stage onwards using Scer\GAL80^ts.αTub84B) results in a strong reduction in the size of the medial part of the leg.

Co-expression of Sp1^{dsRNA.Scer\UAS}, Sp1^GD4097 and Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^Dll-em212 (restricted to second instar stage onwards using Scer\GAL80^ts.αTub84B) results in a strong reduction in the size of the distal part of the leg.

Co-expression of Sp1^{dsRNA.Scer\UAS}, Sp1^GD4097 and Dcr-2^Scer\UAS.cDa under control of Scer\GAL4^btd-MD808 (restricted to second instar stage onwards using Scer\GAL80^ts.αTub84B) results in loss of the antennal a1 and a2 segments.

Co-expression of Sp1^{dsRNA.Scer\UAS}, Sp1^GD4097 and Dcr-2^Scer\UAS.cDa under control of Scer\GAL4^btd-MD808 from embryogenesis onwards results in a reduction in the size of the entire leg, from coxa to the distal tip.

(Estella and Mann, 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808 has tarsal segment phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808, btd^RNAi.UAS has tarsal segment | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808/btd^RNAi.UAS has tarsal segment | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808/btd^RNAi.UAS has leg | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808/btd^RNAi.UAS has third segment of antenna | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808/btd^RNAi.UAS has second segment of antenna | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808/btd^RNAi.UAS has first segment of antenna | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808, btd^RNAi.UAS has leg | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808, btd^RNAi.UAS has third segment of antenna | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808, btd^RNAi.UAS has second segment of antenna | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808, btd^RNAi.UAS has first segment of antenna | heat sensitive phenotype

(Estella et al., 2003)

Scer\GAL4^btd-MD808, Sp1^RNAi.UAS, btd^MD808, btd^RNAi.UAS has tarsal segment phenotype

(Estella et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

When Sp1^{dsRNA.Scer\UAS} is expressed in a btd^MD808 background (driven by the Scer\GAL4^btd-MD808 aspect of the P{GawB}btd^MD808 insertion), at most some reduction in the tarsus is seen in some mutant legs. When both btd^{dsRNA.Scer\UAS} and Sp1^{dsRNA.Scer\UAS} are expressed in a btd^MD808 background (at 29^oC) and driven by the Scer\GAL4^btd-MD808 aspect of the P{GawB}btd^MD808 insertion), the animals are unable to hatch and the antennae and legs are affected in all cases, though to different extents. Segments I and II of the tarsus are often reduced. Leg segments are reduced in size and often fused. When these flies are grown at 17^oC almost no alteration is seen.

(Estella et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Sp1^RNAi.UAS

Sp1^{Scer\UAS.dsRNA}

Sp1^{dsRNA.Scer\UAS}

Sp1^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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