FlyBase Allele Report: Dmel\Tap42[GD8900]

General Information

Symbol

Dmel\Tap42^GD8900

Species

D. melanogaster

Name

FlyBase ID

FBal0208738

Feature type

allele

Associated gene

Dmel\Tap42

Associated Insertion(s)

Carried in Construct

P{GD8900}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8900}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8900

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

decreased occurrence of cell division | third instar larval stage, with Scer\GAL4^ap-md544

(Wang et al., 2012)

increased cell death, with Scer\GAL4^ap-md544

(Wang et al., 2012)

lethal, with Scer\GAL4^Act5C.PI

(Wang et al., 2012)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal, with Scer\GAL4^pnr-MD237

(Wang et al., 2012)

partially lethal - majority die, with Scer\GAL4^ap-md544

(Wang et al., 2012)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^ap-md544

(Wang et al., 2012)

visible, with Scer\GAL4^pnr-MD237

(Wang et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Tap42^GD8900 under the control of Scer\GAL4^Act5C.PI causes complete lethality.

Expression of Tap42^GD8900 under the control of Scer\GAL4^GMR.long does not lead to any apparent defect in the compound eye.

Expression of Tap42^GD8900 under the control of Scer\GAL4^dpp.blk1 does not lead to any apparent phenotype.

Expression of Tap42^GD8900 under the control of Scer\GAL4^pnr-MD237 results in reduced survival rate to adulthood, and survivors display a marked thorax cleft, but no wing defects, as compared to controls.

Expression of Tap42^GD8900 under the control of Scer\GAL4^ap-md544 results in reduced survival rate to adulthood, and survivors display varying degrees of a cleft thorax, completely shrivelled wings, and a subset of these flies display necrosis of the front leg joints, as compared to controls. The wing discs of flies expressing Tap42^GD8900 under the control of Scer\GAL4^ap-md544 show arrest of cell proliferation in the notum region, but no obvious changes in cell proliferation in the wing compartment; and also show increased apoptosis in the wing blade region, as compared to control wing discs.

(Wang et al., 2012)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in extreme defects in the shape of ganglion mother cells in the larval brain.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in extreme defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8900

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, Tap42^GD8900 has visible phenotype, suppressible | partially by mts^XE-2258/mts[+]

(Wang et al., 2012)

Scer\GAL4^ap-md544, Tap42^GD8900 has partially lethal - majority die phenotype, suppressible by mts^XE-2258/mts[+]

(Wang et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^ap-md544, Tap42^GD8900 has visible phenotype, non-suppressible by Scer\GAL4^ap-md544/bsk^DN.UAS.cUa

(Wang et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, Tap42^GD8900 has adult thorax phenotype, suppressible | partially by mts^XE-2258/mts[+]

(Wang et al., 2012)

Scer\GAL4^ap-md544, Tap42^GD8900 has wing | adult stage phenotype, suppressible | partially by mts^XE-2258/mts[+]

(Wang et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^ap-md544, Tap42^GD8900 has adult thorax phenotype, non-suppressible by Scer\GAL4^ap-md544/bsk^DN.UAS.cUa

(Wang et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of bsk^{DN.Scer\UAS.cUa} fails to rescue the cleft thorax phenotype of flies expressing Tap42^GD8900 under the control of Scer\GAL4^ap-md544.

mts^XE-2258/+ partially rescues the cleft thorax and wing phenotypes, and rescues survival of flies expressing Tap42^GD8900 under the control of Scer\GAL4^ap-md544.

(Wang et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Tap42^GD8900 is rescued by Scer\GAL4^ap-md544/Tap42^UAS.cCa

(Wang et al., 2012)

Tap42^GD8900 is rescued by Scer\GAL4^pnr-MD237/Tap42^UAS.cCa

(Wang et al., 2012)

Not rescued by

Tap42^GD8900 is not rescued by Tap42^ED.UAS/Scer\GAL4^ap-md544

(Wang et al., 2012)

Tap42^GD8900 is not rescued by Tap42^ED.UAS/Scer\GAL4^pnr-MD237

(Wang et al., 2012)

Comments

Co-expression of Tap42^Scer\UAS.cCa, but not Tap42^ED.Scer\UAS, rescues the thorax and wing development phenotypes of flies expressing Tap42^GD8900 under the control of Scer\GAL4^ap-md544.

Co-expression of Tap42^Scer\UAS.cCa, but not Tap42^ED.Scer\UAS, rescues the thorax defect and reduced survival of flies expressing Tap42^GD8900 under the control of Scer\GAL4^pnr-MD237.

(Wang et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v25179

w¹¹¹⁸; P{GD8900}v25179/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Linkouts