FlyBase Allele Report: Dmel\Tap42[ED.UAS]

General Information

Symbol

Dmel\Tap42^ED.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0321419

Feature type

allele

Associated gene

Dmel\Tap42

Associated Insertion(s)

Carried in Construct

P{UAS-Tap42.ED}

Key Links

Transgenic product class

dominant_negative_variant

(Wang et al., 2012)

missense_variant

(Wang et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Wang et al., 2012)

missense_variant

(Wang et al., 2012)

Mutagen

in vitro construct

(Wang et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Tap42.ED}

Cytology

Description

UAS regulatory sequences drive expression of a phosphatase binding-defective mutant of Tap42 (contains the amino acid replacements R152E and K155D). The protein may function as a dominant-negative.

(Wang et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tap42

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^ap-md544

(Wang et al., 2012)

visible, with Scer\GAL4^ap-md544

(Wang et al., 2012)

Phenotype Manifest In

mesothoracic tergum, with Scer\GAL4^ap-md544

(Wang et al., 2012)

scutum, with Scer\GAL4^ap-md544

(Wang et al., 2012)

wing | adult stage, with Scer\GAL4^ap-md544

(Wang et al., 2012)

wing vein | adult stage, with Scer\GAL4^ap-md544

(Wang et al., 2012)

Detailed Description

Statement

Reference

Expression of Tap42^ED.Scer\UAS under the control of Scer\GAL4^ap-md544 results in a smaller notum, lacking the scutum, and forked veins in the wings, as compared to controls.

Expression of Tap42^ED.Scer\UAS under the control of Scer\GAL4^pnr-MD237 does not result in any obvious thorax phenotype.

(Wang et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Tap42^ED.UAS/Scer\GAL4^ap-md544 fails to rescue Tap42^GD8900

(Wang et al., 2012)

Tap42^ED.UAS/Scer\GAL4^pnr-MD237 fails to rescue Tap42^GD8900

(Wang et al., 2012)

Comments

Co-expression of Tap42^ED.Scer\UAS fails to rescue the thorax cleft and wing deformities of flies expressing Tap42^GD8900 under the control of Scer\GAL4^ap-md544.

Co-expression of Tap42^ED.Scer\UAS fails to rescue the thorax defect and reduced survival of flies expressing Tap42^GD8900 under the control of Scer\GAL4^pnr-MD237.

(Wang et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tap42^ED.Scer\UAS

Tap42^ED.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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