FlyBase Allele Report: Dmel\aos[GD1666]

General Information

Symbol

Dmel\aos^GD1666

Species

D. melanogaster

Name

FlyBase ID

FBal0209075

Feature type

allele

Associated gene

Dmel\aos

Associated Insertion(s)

Carried in Construct

P{GD1666}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1666}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1666

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chaeta, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

eye, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Dietzl et al., 2007)

Detailed Description

Statement

Reference

Adults expressing aos^GD1666 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in lethality by 7 days after eclosion.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 20-30% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 40-50% or 70-80% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression of argos^GD1666 under the control of Scer\GAL4^Bx-MS1096 leads to crinkled wings.

Expression of argos^GD1666 under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF leads to rough eyes.

(Dietzl et al., 2007)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1666

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, aos^GD1666 has lethal phenotype

(Dietzl et al., 2007)

Scer\GAL4^pnr-MD237, aos^GD1666 has lethal phenotype

(Dietzl et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, aos^GD1666 has eye phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

Other

Statement

Reference

Scer\GAL4^pnr-MD237, aos^GD1666 has thorax phenotype

(Dietzl et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of Dcr-2^Scer\UAS.cDa with argos^GD1666, under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF, results in lethality, which is not seen when only argos^GD1666 is expressed. The eye phenotype of Scer\GAL4^ey.PH;Scer\GAL4^GMR.PF;argos^GD1666 flies is also enhanced when Dcr-2^Scer\UAS.cDa is coexpressed; the rough eye phenotype is more severe and eyes are also glazed or necrotic. In addition, coexpression of argos^GD1666 and Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^pnr-MD237 results in lethality and defects in thorax closure, while Scer\GAL4^pnr-MD237>argos^GD1666 expression alone does not.

(Dietzl et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v3237

w¹¹¹⁸; P{GD1666}v3237

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1666

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

aos^GD1666

argos^GD1666

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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