Expressing bab2^GD17091 under the control of Scer\GAL4^GMR42B09 induces a strong ovary defects, with substantially reduced, disorganized and fused ovariole material.

Expression of bab2^GD17091 RNAi under the control of Scer\GAL4^Act5C.PU (using tub-Gal80[ts] to limit the RNAi expression to the adult stage) results in loss of germline stem cells and GSCs that directly differentiate into spermatogonial cells in adult testes.

Expression of bab2^GD17091 under the control of Scer\GAL4^bab1-Pgal4-2 does not alter the number of somatic gonad precursor (SGP) cells at hatching (L0) compared to controls. However, by the larval-pupal transition stage swarm cell migration appears incomplete and the number of swarm cells is significantly increased compared to controls. A significantly greater proportion of posterior cells is seen at the expense of anterior cells but the proportion of anterior cells that become terminal filament cells is similar to controls. Larvae expressing bab2^GD17091 under the control of Scer\GAL4^bab1-Pgal4-2 ultimately fail to make normal terminal filaments or ovarioles. The body size of the adult flies is comparable to wild type.

Adults expressing bab2^GD17091 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 30-40% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.